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Quorum addiction module

• Inventory of all the parts already ready to use.
• Midiprep on the RBS strenght 1.
• Miniprep on some of the parts needed (AHL degrading enzyme, LuxR, Both promoters, LuxI and RBS strenght 0.3).

Module hydrogen

• After the PCR of verification, we found out that we obtained the ldhA deleted mutant. The other deletion candidate were all negative.
• We continue the purification process for the deletion candidate
• We continue to try to obtain other deletion candidates
• We tested the insertion of tdcE with the RBS.

Module homologous recombination

• We finally obtained the plasmid TOPO with the cm and kan resistance cassettes !
• We can now digest the two resistance cassette in order to insert them in the pSB1C3

Module heavy metals detection

• We inserted the gene of the carotene behind a constitutive promoter
• We inserted the copper sensitive promoter in front of a RFP