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Team:Alberta/Kit components
From 2010.igem.org
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| - | + | [[Image:Alberta box.jpg|right|280px|thumb|GENOMIKON's DNA parts]] | |
| - | + | '''Linker Parts''' | |
*Transcription | *Transcription | ||
*5 Anderson Promoters | *5 Anderson Promoters | ||
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*Short null-linker for inversion of ends | *Short null-linker for inversion of ends | ||
*Fusion Protein Link | *Fusion Protein Link | ||
| + | *Protein Tags | ||
| + | |||
| + | |||
'''Other Parts''' | '''Other Parts''' | ||
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*Caps | *Caps | ||
*Anchors | *Anchors | ||
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| + | <div style="clear:both"></div> | ||
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==Other Components== | ==Other Components== | ||
<div id="horiz-line"></div> | <div id="horiz-line"></div> | ||
| - | These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. | + | These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. |
| + | (All components are pre-sterilized.) | ||
*Precision Droppers to accurately dispense small volumes of liquids | *Precision Droppers to accurately dispense small volumes of liquids | ||
| Line 78: | Line 85: | ||
*Plates | *Plates | ||
*Iron Micro Beads | *Iron Micro Beads | ||
| + | *Thermometer | ||
| + | [[Image:Alberta_Spread.jpg|550px|center|thumb|All the components of GENOMIKON can be seen in this picture.]] | ||
| + | |||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Latest revision as of 02:31, 28 October 2010
The DNA Components
Open Reading Frame Parts
| Fluorescent Proteins | Carotenoid Pathway | Violacein Pathway | Transcription Factors | Resistance Marker |
|---|---|---|---|---|
| GFP | CrtE | VioA | LacI | Ampicillin |
| mRFP | CrtB | VioB | AraC | Chloramphenicol |
| CFP | CrtI | VioD | LambdaCI | Kanamycin |
| YFP | CrtY | VioE | TetR | Tetramycin |
Linker Parts
- Transcription
- 5 Anderson Promoters
- Operon Linker
- Anti-linker as a negative control
- Short null-linker for inversion of ends
- Fusion Protein Link
- Protein Tags
Other Parts
- High, medium and low copy number Oris
- Caps
- Anchors
Other Components
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. (All components are pre-sterilized.)
- Precision Droppers to accurately dispense small volumes of liquids
- Magnetic Racks
- Eppendorf Tubes
- Wash Buffer
- Elution Buffer
- Lyophilized Competent Cells
- LB media
- Plates
- Iron Micro Beads
- Thermometer
