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Team:Alberta/Kit components
From 2010.igem.org
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! Violacein Pathway | ! Violacein Pathway | ||
! Transcription Factors | ! Transcription Factors | ||
| + | ! Resistance Marker | ||
|- | |- | ||
| - | | | + | | GFP |
| CrtE | | CrtE | ||
| VioA | | VioA | ||
| LacI | | LacI | ||
| + | | Ampicillin | ||
|- | |- | ||
| - | | | + | | mRFP |
| CrtB | | CrtB | ||
| VioB | | VioB | ||
| AraC | | AraC | ||
| + | |Chloramphenicol | ||
|- | |- | ||
| - | | | + | | CFP |
| CrtI | | CrtI | ||
| VioD | | VioD | ||
| LambdaCI | | LambdaCI | ||
| + | | Kanamycin | ||
|- | |- | ||
| - | | | + | | YFP |
| CrtY | | CrtY | ||
| VioE | | VioE | ||
| TetR | | TetR | ||
| + | | Tetramycin | ||
|} | |} | ||
| + | [[Image:Alberta box.jpg|right|280px|thumb|GENOMIKON's DNA parts]] | ||
| + | '''Linker Parts''' | ||
| + | *Transcription | ||
| + | *5 Anderson Promoters | ||
| + | *Operon Linker | ||
| + | *Anti-linker as a negative control | ||
| + | *Short null-linker for inversion of ends | ||
| + | *Fusion Protein Link | ||
| + | *Protein Tags | ||
| - | |||
'''Other Parts''' | '''Other Parts''' | ||
| + | *High, medium and low copy number Oris | ||
| + | *Caps | ||
| + | *Anchors | ||
| + | |||
| + | <div style="clear:both"></div> | ||
==Other Components== | ==Other Components== | ||
<div id="horiz-line"></div> | <div id="horiz-line"></div> | ||
| - | These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. | + | These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. |
| + | (All components are pre-sterilized.) | ||
*Precision Droppers to accurately dispense small volumes of liquids | *Precision Droppers to accurately dispense small volumes of liquids | ||
| Line 63: | Line 82: | ||
*Elution Buffer | *Elution Buffer | ||
*Lyophilized Competent Cells | *Lyophilized Competent Cells | ||
| - | * | + | *LB media |
*Plates | *Plates | ||
*Iron Micro Beads | *Iron Micro Beads | ||
| + | *Thermometer | ||
| + | [[Image:Alberta_Spread.jpg|550px|center|thumb|All the components of GENOMIKON can be seen in this picture.]] | ||
| + | |||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Latest revision as of 02:31, 28 October 2010
The DNA Components
Open Reading Frame Parts
| Fluorescent Proteins | Carotenoid Pathway | Violacein Pathway | Transcription Factors | Resistance Marker |
|---|---|---|---|---|
| GFP | CrtE | VioA | LacI | Ampicillin |
| mRFP | CrtB | VioB | AraC | Chloramphenicol |
| CFP | CrtI | VioD | LambdaCI | Kanamycin |
| YFP | CrtY | VioE | TetR | Tetramycin |
Linker Parts
- Transcription
- 5 Anderson Promoters
- Operon Linker
- Anti-linker as a negative control
- Short null-linker for inversion of ends
- Fusion Protein Link
- Protein Tags
Other Parts
- High, medium and low copy number Oris
- Caps
- Anchors
Other Components
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. (All components are pre-sterilized.)
- Precision Droppers to accurately dispense small volumes of liquids
- Magnetic Racks
- Eppendorf Tubes
- Wash Buffer
- Elution Buffer
- Lyophilized Competent Cells
- LB media
- Plates
- Iron Micro Beads
- Thermometer
