Team:Newcastle/25 August 2010

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(Restriction digestion and gel extraction linearized pSB1C3)
(Results and Conclusion)
 
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=First transformation of ''Bacillius subtilis'' 168 with Prrnb-GFP containing ''yneA''=
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=Transformation of ''Bacillius subtilis'' 168 with pGFP-rrnB containing ''yneA''=
==Aim==
==Aim==
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To transform ''yneA'' into competent ''B. subtilis''.
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We performed gel electrophoresis on our 12 digested minipreps and found 8 were the correct product. We transformed ''B. subtilis'' 168 with one of the samples (1).
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[[Image:filamentous_in_pgfprrnb.jpg|800px]]
==Materials and Protocol==
==Materials and Protocol==
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Please refer to results in [[Team:Newcastle/26_August_2010|tomorrow]]'s lab book.
Please refer to results in [[Team:Newcastle/26_August_2010|tomorrow]]'s lab book.
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Latest revision as of 02:04, 28 October 2010

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Contents

Transformation of Bacillius subtilis 168 with pGFP-rrnB containing yneA

Aim

We performed gel electrophoresis on our 12 digested minipreps and found 8 were the correct product. We transformed B. subtilis 168 with one of the samples (1).

Filamentous in pgfprrnb.jpg

Materials and Protocol

Please refer to transformation of B. subtilis.

Results and Conclusion

Please refer to results in tomorrow's lab book.


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