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Team:Queens-Canada/5 August 2010
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(New page: <h2>August 5, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Thai and Basia '''pMec-7''', '''pStr-1''' Program run using 9:1 FAST:HIFI blend wi...) |
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<h2>August 5, 2010</h2> | <h2>August 5, 2010</h2> | ||
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Hao and Basia and Thai | Hao and Basia and Thai | ||
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| + | '''pStr-1''', '''pMec-7''', '''pStr-1::1A2''', '''pSra-10''', '''pHsp-3''' | ||
Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V. | Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V. | ||
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Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C. | Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C. | ||
| + | |||
| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | Practiced injections with Sur-5::GFP marker. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 01:59, 28 October 2010
August 5, 2010
PCR
Thai and Basia
pMec-7, pStr-1
Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.
Gel Electrophoresis
Hao and Basia and Thai
pStr-1, pMec-7, pStr-1::1A2, pSra-10, pHsp-3
Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.
Gel Electrophoresis
Hao and Chris
pRab-7, pSip-1, pStr-1, pHsp-3
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 110 V.
PCR
Chris
pSra-10, pHsp-3
Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.
Microinjection
Hao
Practiced injections with Sur-5::GFP marker.
