Team:Queens-Canada/5 August 2010

From 2010.igem.org

August 5, 2010

PCR

Thai and Basia

pMec-7, pStr-1

Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.

Gel Electrophoresis

Hao and Basia and Thai

pStr-1, pMec-7, pStr-1::1A2, pSra-10, pHsp-3

Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.

Gel Electrophoresis

Hao and Chris

pRab-7, pSip-1, pStr-1, pHsp-3

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 110 V.

PCR

Chris

pSra-10, pHsp-3

Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.

Microinjection

Hao

Practiced injections with Sur-5::GFP marker.