"
Talk:Team:IvyTech-South Bend/21 September 2010
From 2010.igem.org
< Talk:Team:IvyTech-South Bend(Difference between revisions)
Rchamberlin (Talk | contribs) (New page: == 9/21/10 == Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23 1) I will put frozen electro comp cells from -80 degrees C freezer 2) We wil...) |
Rchamberlin (Talk | contribs) (→9/21/10) |
||
| Line 14: | Line 14: | ||
5) then removed with 1 mL SOB media | 5) then removed with 1 mL SOB media | ||
| - | 6) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs | + | 6) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs G T. will streak. |
| + | |||
| + | |||
| + | JH | ||
| + | |||
| + | ==9/21/10== | ||
| + | |||
| + | Pulling of LacZ plate 1 well 23F __ I732094 | ||
| + | I added 10ul of di H2O to the well, did not pull 5 ul out because we did not have electorcom cells ready | ||
| + | There is 10 ul of di H20 and lacZ in well | ||
| + | --[[User:Rchamberlin|Rchamberlin]] 18:49, 26 October 2010 (UTC) for Dgarvey | ||
Latest revision as of 00:51, 28 October 2010
9/21/10
Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23
1) I will put frozen electro comp cells from -80 degrees C freezer
2) We will do two “2” electroporations with (AT) one with 5 mL T9002 Gene, and one with 20 mL
3) Icing Cuvette (.1) and now prepared to electroporate
4) Reading for 20 mL was 2.2 kv @ 0.70 ms
5) then removed with 1 mL SOB media
6) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs G T. will streak.
JH
9/21/10
Pulling of LacZ plate 1 well 23F __ I732094 I added 10ul of di H2O to the well, did not pull 5 ul out because we did not have electorcom cells ready There is 10 ul of di H20 and lacZ in well --Rchamberlin 18:49, 26 October 2010 (UTC) for Dgarvey
