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| | ==Notebook== | | ==Notebook== |
| | + | <html> |
| | + | <style type="text/css"> |
| | + | table.calendar { margin: 0; padding: 10px; } |
| | + | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } |
| | + | table.month .heading td { padding:2px; background-color:#0f6666; color:#00ff00; text-align:center; font-size:120%; font-weight:bold; } |
| | + | table.month .dow td { color:#00ff00; text-align:center; font-size:110%; } |
| | + | table.month td.today { background-color:#ddd;} |
| | + | table.month td { |
| | + | border: none; |
| | + | margin: 0; |
| | + | padding: 1.5pt 2pt; |
| | + | font-weight: bold; |
| | + | font-size: 10pt; |
| | + | text-align: center; |
| | + | background-color: #000000; |
| | + | } |
| | + | #bodyContent table.month a { background:none; padding:0 } |
| | + | .day-active { color:#00FF00 } |
| | + | .day-empty { color:#006666 } |
| | + | </style> |
| | + | </html> |
| | + | {| cellpadding="20" align="center" |
| | + | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }} |
| | + | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }} |
| | + | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }} |
| | + | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }} |
| | + | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }} |
| | + | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }} |
| | + | |} |
| | + | ===IGEM Part #s and Uses=== |
| | | | |
| - | {{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }}
| + | BBa_T9002 – Producer Controler Device–Plate2 Well 9A |
| | | | |
| | + | BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K |
| | | | |
| | + | BBa_F2620– LuxR with terminator– Plate 2 Well 6E |
| | | | |
| | + | BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A |
| | | | |
| - | {{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }}
| + | BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O |
| | + | |
| | + | BBa_pSB1C3—Plasmid—Plate 1 Well 3A |
| | + | |
| | + | BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A |
| | + | |
| | + | BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A |
| | + | |
| | + | == 6/28/10 == |
| | + | Preparing SOB |
| | + | |
| | + | == 6/30/10 == |
| | + | |
| | + | Richard Lab:Electroporation of E. coli |
| | + | |
| | + | == 7/28/10 == |
| | + | |
| | + | Usage and extraction |
| | | | |
| | == 8/24/10 == | | == 8/24/10 == |
| | + | |
| | Today we will be pouring new plates for streaking new transformed cells. | | Today we will be pouring new plates for streaking new transformed cells. |
| | | | |
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| | | | |
| | | | |
| - | We will be running a gel to determine if our DNA sample was successfully electroplated.
| + | We will be running a gel to determine if our DNA sample was successfully electroplated. |
| | | | |
| | == 8/27/10 == | | == 8/27/10 == |
| | | | |
| - | | + | Today we will be electroplating part KBBa_3131010 |
| - | Today we will be electroplating part KBBa_3131010
| + | |
| | | | |
| | == 8/31/10 == | | == 8/31/10 == |
| | | | |
| - | | + | Results of transformation - the plates had growth but (no) distinct colony pattern. |
| - | Results of transformation - the plates had growth but (no) distinct colony pattern.
| + | |
| | | | |
| | == 8/31/10 == | | == 8/31/10 == |
| | | | |
| - | | + | After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH |
| - | After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
| + | |
| | | | |
| | == 9/1/10 == | | == 9/1/10 == |
| | | | |
| - | Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
| + | Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock. |
| | | | |
| | == 9/2/10 == | | == 9/2/10 == |
| - |
| |
| | | | |
| | Results from streaking – | | Results from streaking – |
| | | | |
| | == 9/2/10 == | | == 9/2/10 == |
| - |
| |
| | | | |
| | Protocol For Making LB-Broth/Agar | | Protocol For Making LB-Broth/Agar |
| - |
| |
| - |
| |
| | | | |
| | == 9/3/10 == | | == 9/3/10 == |
| - |
| |
| | | | |
| | Today I’m finishing making the LB/Agar from yesterday. | | Today I’m finishing making the LB/Agar from yesterday. |
| | | | |
| | == 9/9/10 == | | == 9/9/10 == |
| - |
| |
| | | | |
| | Due to conflict we will be changing from E Coli to yeast. | | Due to conflict we will be changing from E Coli to yeast. |
| | | | |
| | == 9/10/10 == | | == 9/10/10 == |
| - |
| |
| | | | |
| | Lux Casette Right Promoter BBa_I1051 | | Lux Casette Right Promoter BBa_I1051 |
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| | == 9/14/10 == | | == 9/14/10 == |
| | | | |
| - | | + | Today we found growth from our Electroporated E – Coli parts |
| - | Today we found growth from our Electroporated E – Coli parts
| + | |
| | | | |
| | == 9/15/10 == | | == 9/15/10 == |
| | | | |
| - | | + | Today we will be extracting DNA from out electroporated cells/T9002 |
| - | - Today we will be extracting DNA from out electroporated cells/T9002
| + | B-galactosidase Assay |
| - | | + | |
| - | | + | |
| | | | |
| | == 9/16/10 == | | == 9/16/10 == |
| | | | |
| | + | Today we will wash |
| | | | |
| - | Today we will wash our electrocomp grown t/pos bacteria with 1.2 mL Glycerol then electroporate
| + | == 9/17/10 == |
| - | Following protocol from page 15
| + | |
| - | First prof. T sterilized the glycerol passing it through the .2 micron filter into 50 mL sterile centrifuge tube
| + | |
| - | 1 – then we added 1 mL 10 percent Glycerol to suspended cells
| + | |
| - | 2 – then centrifuged at 1000 rpm for 10 min
| + | |
| - | - then removed supernate with BR 1000 micropipettor
| + | |
| - | - then add 1 mL 10 percent Glycerol then repeat steps 1 & 2 “4” times then remove sup. and freeze cells at -80 degrees C freezer.
| + | |
| | | | |
| - | - Today we also set up a new IGem work are in back lab look at pic
| + | Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work! |
| | | | |
| | | | |
| - | == 9/17/10 == | + | == 10/18/10 == |
| | | | |
| | + | cutting enzymes |
| | | | |
| - | Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast
| + | == 9/21/10 == |
| - | this will also Work!
| + | |
| | | | |
| - | Following protocols from pgs 15 &19
| + | Today we will electroporate Agrobacterium with part T9002 |
| - | -instead of Electroporating our part we will use PG10 to determine if these lines are good hosts
| + | |
| | | | |
| - | First we will be pouring a full pack of LB/Agar/Amp plates using the LB/Agar I made on 9/2/10 then adding amp made by DG on 9/1/10 we will also add 5 mL Arabonse to the Agar before pouring plates have been poured now letting stand for an hour to harden.
| + | Electrophoresis |
| | | | |
| - | -Now I will be making 1L of LB/Broth for Culturing.
| + | == 9/22/10 == |
| - | Lot 9082989 ex. 2014-02-28
| + | |
| - | BD Difco LB/Broth – Lennox 20 g per 1L
| + | |
| - | so I will put 10 g into 2 elenmyier flask/capped one weighed 10.0023 g and the other weighed 10.0011 g then I added 500 mL into each flask then shook to mix then dissolved in microwaved for 8 oz setting once then placed into Autoclave for 15 min @ 121 degrees C until done.
| + | |
| | | | |
| - | Now we are going electroporate our grown t/pos Electro Comp Cells from yesterday with PG10 using protocol from pg 15 changing # 5 to 5 mL of PG10
| + | Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample |
| - | We will be using a different protocol for electroporation of grown t/pos bacteria from Bio-Rad’s website prof. T will print and I’ll place here.
| + | |
| | | | |
| - | The mycobacterium will be done by electroporation protocol on pg 15.
| + | == 9/22/10 == |
| | | | |
| - | 1) First I placed a Cuvette into Ice bath and thawed electro comp cells
| + | The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens |
| - | 2) I set up 5 1.5 mL Sterile Centrifuge tubes with 900 mL SOB media
| + | |
| - | 3) we will electroporate Irene’s electrocomp cells w/PG10 and mine will contain the parts after electroporation. | + | |
| | | | |
| - | Code
| + | == 9/23/10 == |
| - | AT – A.tumefacions SL – S.lactis BM – B.magetanium MP – Mycobacteria
| + | |
| - | BT – B.thurigensis
| + | |
| | | | |
| - | split cells using 40 mL Hy part my cells then electroporate
| + | Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces. |
| | | | |
| - | I electroporated all except mycobacteria now letting cells sit for at least
| + | ==9/24/10 == |
| - | Just added 1 mL to electroporated Cultures they had sat for 20 min before adding.
| + | |
| - |
| + | |
| - | - Now I will electroporate mycobacteria using E – Coli protocol from pg 15 using 40 mL cells 4 mL pg10 Electroporation hit twice Read out 1.8 kv @ 5.2 ms then 1.79 kv @ 5.1 ms. then placed into 900 mL SOB media then incubate @ 30 degrees C for 3 hrs.
| + | |
| | | | |
| - | Results
| + | Today I’m testing absorbance of our trials @ 395nm |
| - | From this we found that agrobacteria will be the best host for our beast/IGem
| + | |
| - | we will do further testing towards our ultimate goal.
| + | |
| | | | |
| | + | == 9/28/10 == |
| | | | |
| - | == 9/21/10 ==
| + | Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10 |
| | | | |
| | + | == 9/30/10== |
| | + | LacZ without GFP also with RBS and terminator |
| | | | |
| - | Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23
| |
| | | | |
| - | 1) I will put frozen electro comp cells from -80 degrees C freezer | + | ==10/1/10== |
| - | 2) We will do two “2” electroporations with (AT) one with 5 mL T9002 Gene, and one with 20 mL
| + | |
| - | 3) Icing Cuvette (.1) and now prepared to electroporate
| + | |
| - | 4) Reading for 20 mL was 2.2 kv @ 0.70 ms
| + | |
| - | 5) then removed with 1 mL SOB media
| + | |
| - | 6) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs prof T. will streak.
| + | |
| | | | |
| | + | electrophoresis |
| | | | |
| - | == 9/22/10 == | + | ==10/5/10== |
| | | | |
| | + | pTet GFP |
| | | | |
| - | Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew
| + | ==10/7/10== |
| - | not 5 mL sample
| + | |
| - | 1) Scrap bacteria from transformed plate
| + | |
| - | 2) Set up (3) Cuvettes 1 with 200 mL of bacteria + 800 mL LB – Lennox
| + | |
| - | broth 2 with 200 mL of bacteria + 800 mL of A.tumafaciens
| + | |
| - | superant 3 with 200 mL of bacteria + 800 mL of E – Coli Superant
| + | |
| - | 4 with 200 mL LB broth + 800 mL of LB
| + | |
| - | 5 with 200 mL LB broth + 800 mL of A tumafaciens
| + | |
| - | 6 with 200 mL LB broth + 800 mL of E – Coli Superant
| + | |
| - | change 500 mL cells either Agro-T9002 for sample A,B,C or Agro for D,E,F and 500 mL Sup + 200 mL SOB
| + | |
| | | | |
| | + | Electroporating 2 parts with E-coli bacteria |
| | | | |
| - | == 9/22/10 == | + | ==10/8/10== |
| | | | |
| | | | |
| - | The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
| + | LB-Lennox Broth & Electroporation |
| | | | |
| - | == 9/23/10 == | + | ==10/12/10== |
| | + | hold until Friday |
| | | | |
| | + | ==10/13/10== |
| | + | Discoveries! |
| | | | |
| - | Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
| + | ==10/15/10== |
| | | | |
| - | ==9/24/10 ==
| + | Using openwetware.org protocol |
| | | | |
| | + | B-galactosidase Assay |
| | | | |
| - | Today I’m testing absorbance of our trials @ 395nm
| + | BBL Trypticase soy Broth and Agar |
| | | | |
| - | == 9/28/10 ==
| + | DPBS |
| | | | |
| | + | == 10/19/10== |
| | + | Protocol-- Electroporation man for E-Coli |
| | + | == 10/21/10 == |
| | + | High Efficiency Electrotransformation of E.coli |
| | | | |
| - | Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
| + | == 10/25/10 == |
| | | | |
| | + | DNA from BlueGuy |
| | | | |
| | + | == 10/26/10 == |
| | + | Final steps pack and ship |
| | | | |
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| - | |[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]] | + | |align="center"|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]] |
| | |- | | |- |
| | | | | | |
| - | ''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
| |
| | | | |
| | | | | | |
| - | |align="center"|[[Team:IvyTech-South_Bend | Team Example]] | + | | |
| | |} | | |} |
| | | | |
Notebook
| June
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| M | T | W | T | F | S | S
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|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_June_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_June_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_June_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_June_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_June_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_June_2010&action=edit 6]
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_June_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_June_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_June_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_June_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_June_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_June_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_June_2010&action=edit 13]
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_June_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_June_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_June_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_June_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_June_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_June_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_June_2010&action=edit 20]
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_June_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_June_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_June_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_June_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_June_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_June_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_June_2010&action=edit 27]
|
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_June_2010 28]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/29_June_2010 29]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_June_2010 30]
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| July
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| M | T | W | T | F | S | S
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| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_July_2010 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_July_2010&action=edit 4]
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_July_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_July_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_July_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_July_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_July_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_July_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_July_2010&action=edit 11]
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_July_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_July_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_July_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_July_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_July_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_July_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_July_2010&action=edit 18]
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_July_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_July_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_July_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_July_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_July_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_July_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_July_2010&action=edit 25]
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_July_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_July_2010&action=edit 27]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_July_2010 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_July_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_July_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_July_2010&action=edit 31]
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| August
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| M | T | W | T | F | S | S
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_August_2010&action=edit 1]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_August_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_August_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_August_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_August_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_August_2010&action=edit 7]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_August_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_August_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_August_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_August_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_August_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_August_2010&action=edit 14]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_August_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_August_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_August_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_August_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_August_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_August_2010&action=edit 22]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_August_2010&action=edit 23]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_August_2010 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_August_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_August_2010&action=edit 26]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/27_August_2010 27]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_August_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_August_2010&action=edit 29]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_August_2010&action=edit 30]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/31_August_2010 31]
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| September
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| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_September_2010 1]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/2_September_2010 2]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/3_September_2010 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_September_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_September_2010&action=edit 5]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_September_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_September_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_September_2010&action=edit 8]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/9_September_2010 9]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/10_September_2010 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_September_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_September_2010&action=edit 12]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_September_2010&action=edit 13]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_September_2010 14]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_September_2010 15]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/16_September_2010 16]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/17_September_2010 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_September_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_September_2010&action=edit 19]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_September_2010&action=edit 20]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_September_2010 21]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_September_2010 22]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_September_2010 23]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_September_2010 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_September_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_September_2010&action=edit 26]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_September_2010&action=edit 27]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_September_2010 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_September_2010&action=edit 29]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_September_2010 30]
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| October
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| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_October_2010 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_October_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_October_2010&action=edit 3]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_October_2010&action=edit 4]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/5_October_2010 5]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_October_2010 6]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/7_October_2010 7]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/8_October_2010 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_October_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_October_2010&action=edit 10]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_October_2010&action=edit 11]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/12_October_2010 12]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/13_October_2010 13]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_October_2010 14]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_October_2010 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_October_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_October_2010&action=edit 17]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_October_2010&action=edit 18]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/19_October_2010 19]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/20_October_2010 20]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_October_2010 21]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_October_2010 22]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_October_2010 23]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_October_2010 24]
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| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/25_October_2010 25]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/26_October_2010 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_October_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_October_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_October_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_October_2010&action=edit 31]
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| November
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_November_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_November_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_November_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_November_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_November_2010&action=edit 5]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_November_2010 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_November_2010&action=edit 7]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_November_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_November_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_November_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_November_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_November_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_November_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_November_2010&action=edit 14]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_November_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_November_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_November_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_November_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_November_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_November_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_November_2010&action=edit 21]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_November_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_November_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_November_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_November_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_November_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_November_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_November_2010&action=edit 28]
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| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_November_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_November_2010&action=edit 30]
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IGEM Part #s and Uses
BBa_T9002 – Producer Controler Device–Plate2 Well 9A
BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
BBa_F2620– LuxR with terminator– Plate 2 Well 6E
BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
6/28/10
Preparing SOB
6/30/10
Richard Lab:Electroporation of E. coli
7/28/10
Usage and extraction
8/24/10
Today we will be pouring new plates for streaking new transformed cells.
8/27/10
We will be running a gel to determine if our DNA sample was successfully electroplated.
8/27/10
Today we will be electroplating part KBBa_3131010
8/31/10
Results of transformation - the plates had growth but (no) distinct colony pattern.
8/31/10
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
9/1/10
Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
9/2/10
Results from streaking –
9/2/10
Protocol For Making LB-Broth/Agar
9/3/10
Today I’m finishing making the LB/Agar from yesterday.
9/9/10
Due to conflict we will be changing from E Coli to yeast.
9/10/10
Lux Casette Right Promoter BBa_I1051
9/14/10
Today we found growth from our Electroporated E – Coli parts
9/15/10
Today we will be extracting DNA from out electroporated cells/T9002
B-galactosidase Assay
9/16/10
Today we will wash
9/17/10
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
10/18/10
cutting enzymes
9/21/10
Today we will electroporate Agrobacterium with part T9002
Electrophoresis
9/22/10
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
9/22/10
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
9/23/10
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
9/24/10
Today I’m testing absorbance of our trials @ 395nm
9/28/10
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
9/30/10
LacZ without GFP also with RBS and terminator
10/1/10
electrophoresis
10/5/10
pTet GFP
10/7/10
Electroporating 2 parts with E-coli bacteria
10/8/10
LB-Lennox Broth & Electroporation
10/12/10
hold until Friday
10/13/10
Discoveries!
10/15/10
Using openwetware.org protocol
B-galactosidase Assay
BBL Trypticase soy Broth and Agar
DPBS
10/19/10
Protocol-- Electroporation man for E-Coli
10/21/10
High Efficiency Electrotransformation of E.coli
10/25/10
DNA from BlueGuy
10/26/10
Final steps pack and ship
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