Team:Newcastle/20 August 2010

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=Aims=
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=Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3=
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Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.
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==Aims==
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==Materials and protocol==
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To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing ''yneA'' with pGFPrrnB and pSB1C3 from[[Team:Newcastle/19_August_2010|yesterday]].
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Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]], [[Team:Newcastle/Gel_extraction| gel extraction]] and [[TeamNewcastleNanoDrop_Spectrophotometer| NanoDrop]] protocols. '''Vacuum manifold''' will be used.
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=Gel Extraction=
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==Aims==
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To purify the PCR products of digested ''yneA'', pGFPrrnB and pSB1C3 from [[Team:Newcastle/19_August_2010|yesterday]].
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==Materials and Protocol==
==Materials and Protocol==
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Please refer to [[Team:Newcastle/Gel_extraction|gel extraction]].
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Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
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{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 00:33, 28 October 2010

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Transformation of ligated yneA into pGFPrrnB and pSB1C3

Aims

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

Materials and Protocol

Please refer to transformation of E. coli.


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