Team:Newcastle/20 August 2010

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(New page: v=Subtilin Immunity= ==Aims== We have modified the promoters for Plasmid Vector (part 1), Promoter & RBS (part 2) and Double terminator (part 4), thus we aim to do Phusion PCR today. =...)
 
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=Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3=
==Aims==
==Aims==
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We have modified the promoters for Plasmid Vector (part 1), Promoter & RBS (part 2) and Double terminator (part 4), thus we aim to do Phusion PCR today.  
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To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing ''yneA'' with pGFPrrnB and pSB1C3 from[[Team:Newcastle/19_August_2010|yesterday]].
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==Materials and Protocol==
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Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
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==Materials and protocol==
 
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Please refer to  [[Team:Newcastle/PCR| PCR]] for Phusion PCR protocol.
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{{Team:Newcastle/footer}}

Latest revision as of 00:33, 28 October 2010

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Transformation of ligated yneA into pGFPrrnB and pSB1C3

Aims

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

Materials and Protocol

Please refer to transformation of E. coli.


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