Team:Newcastle/20 August 2010

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(Difference between revisions)
(→Plating Bacillus subtilis BFS678)
(→Miniprep for yneA, pGFPrrnB and pSB1C3)
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=Miniprep for ''yneA'', pGFPrrnB and pSB1C3=
 
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==Aim==
 
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The aim of this experiment is to produce stocks of ''yneA'', pGFPrrnB and pSB1C3.
 
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==Materials and Protocol==
 
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Please refer to [[Team:Newcastle/Qiagen_Minipreps|miniprep]] and [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]].
 
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==Results==
 
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{|border=1
 
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|-
 
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! Sample no.
 
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! ''yneA''
 
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! pGFPrrnB
 
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! pSB1C3
 
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|-
 
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!1
 
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!432.5
 
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!238.9
 
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!126.1
 
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|-
 
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!2
 
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!347.6
 
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!230.7
 
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!107.6
 
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|-
 
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!3
 
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!380.2
 
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!390.9
 
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!121.5
 
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|-
 
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!4
 
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!377.9
 
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!236.2
 
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!112.8
 
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|}
 
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'''Table 1''': Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.
 
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===Discussion===
 
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Results from the NanoDrop show concentration values of more than 100 ng/µl, which means we have obtained good concentration of DNA from the miniprep.
 
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===Conclusion===
 
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We keep the miniprep products as stocks for future use.
 

Revision as of 00:21, 28 October 2010

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Contents

Transformation of ligated yneA into pGFPrrnB and pSB1C3

Aims

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

Materials and Protocol

Please refer to transformation of E. coli.


Plating Bacillus subtilis BFS678

Bacillus subtilis BFS678 has the pMutin4 (and thus lacI) integrated into the chromosome which will be useful for characterisation. Therefore we will be using this strain for transformation with our filamentous cell and arginase BioBricks.


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