Team:SDU-Denmark/project-i

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Our approach focuses on ''E. coli'' strain MG1655 that will be modified to overexpress flagella, in an attempt to increase the force generation potential. We also want to be able to regulate the flow that is generated with a light sensor, that integrates into the chemotaxis pathway, giving us very fast response times. Finally for the photosensor to function properly we will need to introduce retinal biosynthesis to the system. In this way we can avoid altering the buffer solution flowing through the system, this though result in trace amounts of waste products from the cells metabolism.<br><br>
Our approach focuses on ''E. coli'' strain MG1655 that will be modified to overexpress flagella, in an attempt to increase the force generation potential. We also want to be able to regulate the flow that is generated with a light sensor, that integrates into the chemotaxis pathway, giving us very fast response times. Finally for the photosensor to function properly we will need to introduce retinal biosynthesis to the system. In this way we can avoid altering the buffer solution flowing through the system, this though result in trace amounts of waste products from the cells metabolism.<br><br>
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[[Image:Team-sdu-denmark-system overview.png | 300px|thumb|right|An overview of the system components. Part K274210 was produced and charactarized by the Cambridge 2009 team.]]
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[[Image:Team-sdu-denmark-system overview.png |400px|thumb|center|'''Figure 2:'''An overview of our system components. Part K274210 was produced and charactarized by the Cambridge 2009 team.]]
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== Hyperflagellation ==
== Hyperflagellation ==
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= References =
= References =
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[1] Angelani L, Di Leonardo R, Ruocco G, [http://prl.aps.org/abstract/PRL/v102/i4/e048104 Self-starting micromotors in a bacterial bath]. Phys Rev Lett (2009) 102:048104.<br>
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# Angelani L, Di Leonardo R, Ruocco G, [http://prl.aps.org/abstract/PRL/v102/i4/e048104 Self-starting micromotors in a bacterial bath]. Phys Rev Lett (2009) 102:048104.<br>
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[2] Steager E, Kim CB, Patel J, Bith S, Naik C, Reber L, Kim MJ, [http://apl.aip.org/resource/1/applab/v90/i26/p263901_s1 Control of microfabricated structures powered by flagellated bacteria using phototaxis], Appl. Phys. Lett. 90, 263901 (2007), DOI:10.1063/1.2752721<br>
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# Steager E, Kim CB, Patel J, Bith S, Naik C, Reber L, Kim MJ, [http://apl.aip.org/resource/1/applab/v90/i26/p263901_s1 Control of microfabricated structures powered by flagellated bacteria using phototaxis], Appl. Phys. Lett. 90, 263901 (2007), DOI:10.1063/1.2752721<br>
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[3] Darnton N, Turner L, Breuer KS, Berg HC, [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304020 Moving Fluid with Bacterial Carpet], Biophys J. 2004 March; 86(3): 1863–1870.<br>
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# Darnton N, Turner L, Breuer KS, Berg HC, [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304020 Moving Fluid with Bacterial Carpet], Biophys J. 2004 March; 86(3): 1863–1870.<br>
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[4] Kim MJ, Breuer KS,[http://onlinelibrary.wiley.com/doi/10.1002/smll.200700641/abstract Microfluidic pump powered by self-organizing bacteria.] Small 4, 111 (2008).<br>
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# Kim MJ, Breuer KS,[http://onlinelibrary.wiley.com/doi/10.1002/smll.200700641/abstract Microfluidic pump powered by self-organizing bacteria.] Small 4, 111 (2008).<br>
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[5] Martel S, Tremblay CC, Ngakeng S, Langlois G, (2006) [http://apl.aip.org/resource/1/applab/v89/i23/p233904_s1?isAuthorized=no Controlled manipulation and actuation of micro-objects with magnetotactic bacteria], Appl. Phys. Lett. 89, 233904 (2006); doi:10.1063/1.2402221<br>
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# Martel S, Tremblay CC, Ngakeng S, Langlois G, (2006) [http://apl.aip.org/resource/1/applab/v89/i23/p233904_s1?isAuthorized=no Controlled manipulation and actuation of micro-objects with magnetotactic bacteria], Appl. Phys. Lett. 89, 233904 (2006); doi:10.1063/1.2402221<br>
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[6] Steager E, Kim CB, Patel J, Bith S, Naik C, Reber L, Kim MJ, [http://apl.aip.org/resource/1/applab/v90/i26/p263901_s1?isAuthorized=no Control of microfabricated structures powered by flagellated bacteria using phototaxis], Appl. Phys. Lett. 90, 263901 (2007); doi:10.1063/1.2752721<br>
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# Steager E, Kim CB, Patel J, Bith S, Naik C, Reber L, Kim MJ, [http://apl.aip.org/resource/1/applab/v90/i26/p263901_s1?isAuthorized=no Control of microfabricated structures powered by flagellated bacteria using phototaxis], Appl. Phys. Lett. 90, 263901 (2007); doi:10.1063/1.2752721<br>
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[7] Chilcott GS, Hughes TK,[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99010/?tool=pubmed Coupling of Flagellar Gene Expression to Flagellar Assembly in Salmonella enterica Serovar Typhimurium and Escherichia coli], Microbiol Mol Biol Rev. 2000 December; 64(4): 694–708.<br>
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# Chilcott GS, Hughes TK,[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99010/?tool=pubmed Coupling of Flagellar Gene Expression to Flagellar Assembly in Salmonella enterica Serovar Typhimurium and Escherichia coli], Microbiol Mol Biol Rev. 2000 December; 64(4): 694–708.<br>
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[8] Berg HC, [http://www.annualreviews.org/eprint/cDJrS190m62mDRwHrlp9/full/10.1146/annurev.biochem.72.121801.161737 The rotary motor of bacterial flagella], Annual Review of Biochemistry Vol. 72: 19-54 (2003)<br>
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# Berg HC, [http://www.annualreviews.org/eprint/cDJrS190m62mDRwHrlp9/full/10.1146/annurev.biochem.72.121801.161737 The rotary motor of bacterial flagella], Annual Review of Biochemistry Vol. 72: 19-54 (2003)<br>
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[9] Barker CS, Prüß BM,Matsumura P, [http://jb.asm.org/cgi/content/full/186/22/7529?view=long&pmid=15516564 Increased Motility of Escherichia coli by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon], Journal of Bacteriology, November 2004, p. 7529-7537, Vol. 186, No. 22<br>
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# Barker CS, Prüß BM,Matsumura P, [http://jb.asm.org/cgi/content/full/186/22/7529?view=long&pmid=15516564 Increased Motility of Escherichia coli by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon], Journal of Bacteriology, November 2004, p. 7529-7537, Vol. 186, No. 22<br>
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[10] Jung K-H, Spudich EN, Trivedi VD, Spudich JL, [http://jb.asm.org/cgi/content/short/183/21/6365 An Archaeal Photosignal-Transducing Module Mediates Phototaxis in Escherichia coli], Journal of Bacteriology, November 2001, p. 6365-6371, Vol. 183, No. 21<br>
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# Jung K-H, Spudich EN, Trivedi VD, Spudich JL, [http://jb.asm.org/cgi/content/short/183/21/6365 An Archaeal Photosignal-Transducing Module Mediates Phototaxis in Escherichia coli], Journal of Bacteriology, November 2001, p. 6365-6371, Vol. 183, No. 21<br>
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[11] von Lintig J, Vogt K, [http://www.jbc.org/content/275/16/11915 Filling the Gap in Vitamin A Research: Molecular Identification of An Enzyme Cleaving Beta-carotene to Retinal] Journal of Biological Chemistry (ASBMB) 275 (16): 11915–11920 (2000)<br>
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# von Lintig J, Vogt K, [http://www.jbc.org/content/275/16/11915 Filling the Gap in Vitamin A Research: Molecular Identification of An Enzyme Cleaving Beta-carotene to Retinal] Journal of Biological Chemistry (ASBMB) 275 (16): 11915–11920 (2000)<br>
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Latest revision as of 00:05, 28 October 2010