Team:Newcastle/16 August 2010

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=Rehydrating ''yneA''=
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=Hydration of synthesised filametous cell part=
==Aim==
==Aim==
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To get ''yneA'' hydrated so that we can run restriction digest (see below).
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To rehydrate the synthesised filametous cell part.
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==Methods==
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==Matherials and protocol==
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# The dehydrated DNA is resuspended in 0.5 ml of water.
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# The dehydrated DNA is resuspended in 0.5 ml of distilled water.
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# We then find out the concentration of resuspended ''yneA'' from [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]].
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=Transformation of ''E. coli''=
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=Transformation of filametous cell part into ''E. coli'' DH5α=
==Aim==
==Aim==
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Transforming ''yneA'' into ''E. coli'' so that we have plenty of stocks to future tests.
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The aim of the experiment is to transform the synthesised filametous cell part into DH5α to obtain stock.
==Materials and Protocol==
==Materials and Protocol==

Latest revision as of 23:46, 27 October 2010

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Contents

Hydration of synthesised filametous cell part

Aim

To rehydrate the synthesised filametous cell part.

Matherials and protocol

  1. The dehydrated DNA is resuspended in 0.5 ml of distilled water.

Transformation of filametous cell part into E. coli DH5α

Aim

The aim of the experiment is to transform the synthesised filametous cell part into DH5α to obtain stock.

Materials and Protocol

Please refer to transformation of E. coli

Results and Conclusion

Please refer to 17.08.10.

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