Team:Heidelberg/Project/Capsid Shuffling/Homology Based
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==Randomly picked clones== | ==Randomly picked clones== | ||
In parallel, we picked 48 randomly selected clones from the AAV library, packaged them with a YFP construct and tested them using flow cytometry on HepG2, Huh-7 and mouse primary hepatocytes. One clone of those was able to infect both HepG2 and Huh-7 cells, and was thus packaged with a luciferase construct and injected into mice. Mice experiment showed a specific targeting into the liver. | In parallel, we picked 48 randomly selected clones from the AAV library, packaged them with a YFP construct and tested them using flow cytometry on HepG2, Huh-7 and mouse primary hepatocytes. One clone of those was able to infect both HepG2 and Huh-7 cells, and was thus packaged with a luciferase construct and injected into mice. Mice experiment showed a specific targeting into the liver. | ||
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+ | [[Image:hepg2_transyfp_1.tif|350px]] | ||
+ | [[Image:huh7_transyfp1.tif|350px]] | ||
+ | [[Image:facs_43_huh.bmp|350px]] | ||
+ | [[Image:facs_nc_huh7.bmp|350px]] | ||
+ | [[Image:facs_nc_hepg2]] | ||
{{:Team:Heidelberg/Pagemiddle}} | {{:Team:Heidelberg/Pagemiddle}} | ||
{{:Team:Heidelberg/Bottom}} | {{:Team:Heidelberg/Bottom}} |
Revision as of 23:18, 27 October 2010
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