Team:Newcastle/9 August 2010

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{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
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=Transformation of pVeg and lacI=  
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=Subtilin immunity part=
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==Results/Aims==
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Today we were working on our subtilin immunity part cloning strategy.
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* pVeg was successfully transformed - From this we will set up an overnight broth culture for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]]
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* ''lacI'' - To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
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=Transformation of lacI=
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==Aims==
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To transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]].
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==Materials and Protocol==
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Please refer to [[TeamNewcastleTransformation_of_E._coli|Transformation of E.coli]].
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==Result==
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Please refer to [[Team:Newcastle/10_August_2010|10.08.10]] for result.
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{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 23:03, 27 October 2010

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Subtilin immunity part

Today we were working on our subtilin immunity part cloning strategy.

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