Team:Newcastle/9 August 2010

From 2010.igem.org

(Difference between revisions)
(→Results/Aims)
 
(63 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
=Transformation=  
+
=Subtilin immunity part=
-
==Results/Aims==
+
Today we were working on our subtilin immunity part cloning strategy.
-
 
+
-
* pVeg was successfully transformed- From this we will set up an overnight broth culture for plasmid extraction
+
-
* ''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
+
-
 
+
-
=Transformation of lacI=
+
-
 
+
-
==Aims==
+
-
 
+
-
To transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]].
+
-
 
+
-
==Materials and Protocol==
+
-
 
+
-
Please refer to [[TeamNewcastleTransformation_of_E._coli|Transformation of E.coli]].
+
-
 
+
-
==Result==
+
-
 
+
-
Please refer to [[Team:Newcastle/10_August_2010|10.08.10]] for result.
+
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 23:03, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Subtilin immunity part

Today we were working on our subtilin immunity part cloning strategy.

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon
Retrieved from "http://2010.igem.org/Team:Newcastle/9_August_2010"