Team:TU Delft/30 June 2010 content

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Revision as of 18:55, 12 July 2010

Lab Work

We harvested the 50 mL bacterial cells of the 14 BioBricks. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation.

Pseudomonas Putida GPO1 is growing 1 day on different substrates. We measured absorbance of the Pseudomonas Putida to see on which conditions the strain survives. Absorbance measurements

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 ===Lab Work===
-We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]].
+We harvested the 50 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=28_June_2010 14 BioBricks]. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]].
-At the same time we are making more competent cells. We go through our competent cells so quickly.
+''Pseudomonas Putida'' GPO1 is growing 1 day on [https://2010.igem.org/Team:TU_Delft#/blog?blog=29_June_2010 different substrates]. We measured absorbance of the ''Pseudomonas Putida'' to see on which conditions the strain survives.  [https://2010.igem.org/Team:TU_Delft#/blog?blog=1_July_2010| Absorbance measurements]