Team:UCL London/Our BioBricks

From 2010.igem.org

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{{:Team:UCL_London/templates/v2/headerFullWidth}} __NOTOC__
{{:Team:UCL_London/templates/v2/headerFullWidth}} __NOTOC__
 +
='''OUR BIOBRICKS'''=
-
==NEW PARTS CHARACTERISED==
+
The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. Also, it's worth mentioning that we are the very first iGEM team to ever have '''1.2 kg of biomass'''!!
-
The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. This does not necessary mean that they were all incorporated into the final product!
+
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<style type="text/css">
<style type="text/css">
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{| class="datatable"
 
-
|-
 
-
! Part Code
 
-
! Part Name
 
-
! Location
 
-
! Backbone
 
-
! Length(bp)
 
-
|-
 
-
| R0010
 
-
| PROMOTER (lacI Regulated)
 
-
| Plate1_1D
 
-
| pSB1A2
 
-
| 200
 
-
|-
 
-
| R0010
 
-
| PROMOTER (lacI Regulated)
 
-
| Plate1_1D
 
-
| pSB1A2
 
-
| 200
 
-
|}
 
-
==EXISTING PARTS/DEVICES CHARACTERISED==
+
==NEW PARTS SUBMITTED & CHARACTERISED==
-
 
+
-
{| class="datatable"
+
-
|-
+
-
! Part Code
+
-
! Part Name
+
-
! Location
+
-
! Backbone
+
-
! Length(bp)
+
-
|-
+
-
| R0010
+
-
| PROMOTER (lacI Regulated)
+
-
| Plate1_1D
+
-
| pSB1A2
+
-
| 200
+
-
|}
+
-
 
+
-
==Parts Submitted==
+
{| class="datatable"
{| class="datatable"
Line 101: Line 64:
! Length(bp)
! Length(bp)
|-
|-
-
| BBa_K301001
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301000 BBa_K301000]
| Regulatory  
| Regulatory  
| Tac promoter, hybrid derived from trp and lac promoters
| Tac promoter, hybrid derived from trp and lac promoters
Line 107: Line 70:
| 73
| 73
|-
|-
-
| BBa_K301001
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301001 BBa_K301001]
| Device
| Device
| pTAC with RFP reporter
| pTAC with RFP reporter
| Xiang Chen
| Xiang Chen
| 942
| 942
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301003 BBa_K301003]
 +
| Regulatory
 +
| T7 promoter
 +
| Xiang Chen
 +
| 23
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301005 BBa_K301005]
 +
| Coding
 +
| T7 RNA Polymerase
 +
| Xiang Chen
 +
| 1000
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301006 BBa_K301006]
 +
| Device
 +
| Low Oxygen Level Self-induction Genetic Circuit. NarK promoter
 +
| Xiang Chen
 +
| 3082
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301007 BBa_K301007]
 +
| Device
 +
| Low Oxygen Level Self-induction Genetic Circuit. mNarK promoter
 +
| Xiang Chen
 +
| 3032
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301008 BBa_K301008]
 +
| Device
 +
| Self-induction Genetic Circuit with an Inducible Promoter
 +
| Xiang Chen
 +
| 3016
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K301010 BBa_K301010]
 +
| Composite
 +
| Self-induction Genetic Circuit
 +
| Xiang Chen
 +
| 2937
|}
|}
 +
Check out our parts page to see the parts submitted in more detail [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=UCL_London&Done=1|'''UCL_London 2010 iGEM Team Parts'''].
-
Check out our parts page to see the parts submitted in more detail [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=UCL_London&Done=1|UCL_London 2010 iGEM Team Parts].
+
==EXISTING PARTS/DEVICES CHARACTERISED==
-
==OTHER PARTS/DEVICES USED==
+
{| class="datatable"
 +
|-
 +
! Part Code
 +
! Part Name
 +
! Location
 +
! Backbone
 +
! Length(bp)
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K239011 BBa_K239011]
 +
| Stress Light GFP Anaerobic Metabolism Detector 2
 +
|
 +
|
 +
| 972
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K239009 BBa_K239009]
 +
| GFP test devise for Spy promoter
 +
|
 +
|
 +
| 1045
 +
|-
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K239015 BBa_K239015]
 +
| GFP test devise for DegP promoter
 +
|
 +
|
 +
| 1116
 +
|}
 +
 
 +
==OTHER PARTS/DEVICES TRIED/USED==
{| class="datatable"
{| class="datatable"
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! Length(bp)
! Length(bp)
|-
|-
-
| R0010
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]
| PROMOTER (lacI Regulated)
| PROMOTER (lacI Regulated)
| Plate1_1D
| Plate1_1D
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| 200
| 200
|-
|-
-
| K239005
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K239005 BBa_K239005]
| Nark
| Nark
| Eurogentec
| Eurogentec
-
| Amp
+
| pUC57
| 139
| 139
|-
|-
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| K239006
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K239006 BBa_K239006]
| mNark
| mNark
| Eurogentec
| Eurogentec
-
| Amp
+
| pUC57
| 89
| 89
|-
|-
-
| R0079
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079]
| Promoter (LasR & PAI regulated)
| Promoter (LasR & PAI regulated)
| Plate1_19P
| Plate1_19P
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| 936
| 936
|-
|-
-
| K274100
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 BBa_K274100]
| VRDP
| VRDP
| Plate3_6H
| Plate3_6H
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| 3385
| 3385
|-  
|-  
-
| B0015
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
| Double Terminator
| Double Terminator
| Plate1_23L
| Plate1_23L
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| 129
| 129
|-
|-
-
| pSB1K3
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| [http://partsregistry.org/wiki/index.php?title=pSB1K3 pSB1K3]
-
| Kanamycin Resistance
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| Kanamycin Resistance Backbone
| Plate1_5A
| Plate1_5A
| pSB1K3+J04450 insert
| pSB1K3+J04450 insert
| 2206
| 2206
|-
|-
-
| pSB1T3
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| [http://partsregistry.org/wiki/index.php?title=pSB1T3 pSB1T3]
-
| Tetracycline Resistance
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| Tetracycline Resistance Backbone
| Plate 1_7A
| Plate 1_7A
| pSB1T3+J04450 insert
| pSB1T3+J04450 insert
| 2463
| 2463
|-
|-
-
| pSB1C3
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| [http://partsregistry.org/wiki/index.php?title=pSB1C3 pSB1C3]
-
| Chloramphenicol Resistance
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| Chloramphenicol Resistance Backbone
| Plate 1_3A
| Plate 1_3A
| pSB1C3+J04450 insert
| pSB1C3+J04450 insert
| 2072
| 2072
|-
|-
-
| R0011
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011]
| Promoter (lacI regulated, lambda pL hybrid)
| Promoter (lacI regulated, lambda pL hybrid)
| Plate1_6G
| Plate1_6G
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| 55
| 55
|-
|-
-
| B0034
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
| RBS
| RBS
| Plate1_2M
| Plate1_2M
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| 12
| 12
|-
|-
-
| C0079
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0079 BBa_C0079]
| lasR activator from P. aeruginosa PAO1(+LVA)
| lasR activator from P. aeruginosa PAO1(+LVA)
| Plate1_14J
| Plate1_14J
Line 199: Line 226:
| 756
| 756
|-
|-
-
| pSB1AK3
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| [http://partsregistry.org/wiki/index.php?title=pSB1AK3 pSB1AK3]
| Amp+Kana
| Amp+Kana
| Plate1_11A
| Plate1_11A
Line 205: Line 232:
| 3189
| 3189
|-
|-
-
| pSB1AT3
+
| [http://partsregistry.org/wiki/index.php?title=pSB1AT3 pSB1AT3]
| Amp+Tetra
| Amp+Tetra
| Plate1_13A
| Plate1_13A
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| 3446
| 3446
|-
|-
-
| pSB1AC3
+
| [http://partsregistry.org/wiki/index.php?title=pSB1AC3 pSB1AC3]
| Amp+Chlora
| Amp+Chlora
| Plate1_9A
| Plate1_9A
Line 217: Line 244:
| 3055
| 3055
|-
|-
-
| K274200
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 BBa_K274200]
| CrtEBIY with rbs (Orange)
| CrtEBIY with rbs (Orange)
| Plate3_6L
| Plate3_6L
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| 4555
| 4555
|-
|-
-
| K274002
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 BBa_K274002]
| Purple
| Purple
| Plate3_12B
| Plate3_12B
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| 7345
| 7345
|-
|-
-
| K274003
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 BBa_K274003]
| Dark Green
| Dark Green
| Plate3_20H
| Plate3_20H
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| 6032
| 6032
|-
|-
-
| row 15, cell 1
+
|
-
| row 15, cell 2
+
|
| Plate3_20D
| Plate3_20D
| pSB1T3
| pSB1T3
| 6032
| 6032
|-
|-
-
| K274004
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 BBa_K274004]
| Light Green  
| Light Green  
| Plate3_20J
| Plate3_20J
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| 6200
| 6200
|-
|-
-
| I712069
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507 BBa_I13507]
-
| RBS+T7 RNAP
+
| RBS+RFP+Terminator
-
| Plate2_13K
+
-
| pSB1AK3
+
-
|
+
-
|-
+
-
| I719005
+
-
| T7 promoter
+
-
| Plate1_15N
+
-
| pSB1A2
+
-
|
+
-
|-
+
-
| I712074
+
-
| T7 strong pro
+
-
| Plate1_6N
+
-
| pSB1AK8
+
-
|
+
-
|-
+
-
| I13507
+
-
| RBS+RFP+T
+
| Pate1_22O
| Pate1_22O
| pSB1A2
| pSB1A2
| 861
| 861
-
|-
 
|}
|}

Latest revision as of 22:42, 27 October 2010

UCL IGEM 2010

RETURN TO IGEM 2010

OUR BIOBRICKS

The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. Also, it's worth mentioning that we are the very first iGEM team to ever have 1.2 kg of biomass!!

NEW PARTS SUBMITTED & CHARACTERISED

Part Name Part Type Description Designer Length(bp)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301000 BBa_K301000] Regulatory Tac promoter, hybrid derived from trp and lac promoters Xiang Chen 73
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301001 BBa_K301001] Device pTAC with RFP reporter Xiang Chen 942
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301003 BBa_K301003] Regulatory T7 promoter Xiang Chen 23
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301005 BBa_K301005] Coding T7 RNA Polymerase Xiang Chen 1000
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301006 BBa_K301006] Device Low Oxygen Level Self-induction Genetic Circuit. NarK promoter Xiang Chen 3082
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301007 BBa_K301007] Device Low Oxygen Level Self-induction Genetic Circuit. mNarK promoter Xiang Chen 3032
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301008 BBa_K301008] Device Self-induction Genetic Circuit with an Inducible Promoter Xiang Chen 3016
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301010 BBa_K301010] Composite Self-induction Genetic Circuit Xiang Chen 2937

Check out our parts page to see the parts submitted in more detail [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=UCL_London&Done=1|UCL_London 2010 iGEM Team Parts].

EXISTING PARTS/DEVICES CHARACTERISED

Part Code Part Name Location Backbone Length(bp)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239011 BBa_K239011] Stress Light GFP Anaerobic Metabolism Detector 2 972
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239009 BBa_K239009] GFP test devise for Spy promoter 1045
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239015 BBa_K239015] GFP test devise for DegP promoter 1116

OTHER PARTS/DEVICES TRIED/USED

Part Code Part Name Location Backbone Length(bp)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010] PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239005 BBa_K239005] Nark Eurogentec pUC57 139
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239006 BBa_K239006] mNark Eurogentec pUC57 89
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079] Promoter (LasR & PAI regulated) Plate1_19P pSB1A2 936
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 BBa_K274100] VRDP Plate3_6H pSB1A2 3385
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] Double Terminator Plate1_23L pSB1AK3 129
[http://partsregistry.org/wiki/index.php?title=pSB1K3 pSB1K3] Kanamycin Resistance Backbone Plate1_5A pSB1K3+J04450 insert 2206
[http://partsregistry.org/wiki/index.php?title=pSB1T3 pSB1T3] Tetracycline Resistance Backbone Plate 1_7A pSB1T3+J04450 insert 2463
[http://partsregistry.org/wiki/index.php?title=pSB1C3 pSB1C3] Chloramphenicol Resistance Backbone Plate 1_3A pSB1C3+J04450 insert 2072
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011] Promoter (lacI regulated, lambda pL hybrid) Plate1_6G pSB1A2 55
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] RBS Plate1_2M pSB1A2 12
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0079 BBa_C0079] lasR activator from P. aeruginosa PAO1(+LVA) Plate1_14J pSB2K3 756
[http://partsregistry.org/wiki/index.php?title=pSB1AK3 pSB1AK3] Amp+Kana Plate1_11A +J04450 insert 3189
[http://partsregistry.org/wiki/index.php?title=pSB1AT3 pSB1AT3] Amp+Tetra Plate1_13A +J04450 insert 3446
[http://partsregistry.org/wiki/index.php?title=pSB1AC3 pSB1AC3] Amp+Chlora Plate1_9A +J04450 insert 3055
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 BBa_K274200] CrtEBIY with rbs (Orange) Plate3_6L pSB1A2 4555
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 BBa_K274002] Purple Plate3_12B pSB1T3 7345
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 BBa_K274003] Dark Green Plate3_20H pSB1K3 6032
Plate3_20D pSB1T3 6032
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 BBa_K274004] Light Green Plate3_20J pSB1K3 6200
Plate3_22F pSBT3 6200
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507 BBa_I13507] RBS+RFP+Terminator Pate1_22O pSB1A2 861


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