Team:Caltech/Parts

From 2010.igem.org

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== Favorite Bricks ==
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= Favorite Bricks & Characterization Data =
Heat shock promoter: [[BBa_K338001]]
Heat shock promoter: [[BBa_K338001]]
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Plastic, part 2: [[Team:Caltech/BBa_K338004|BBa_K338004]]
Plastic, part 2: [[Team:Caltech/BBa_K338004|BBa_K338004]]
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== All Bricks==
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= All Bricks=
<!-- Following code generated by CompletedPartsFormatter, (c) 2010 Alexandre Boulgakov -->
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If your iGEM team is interested in any of our unsubmitted parts, let us know and we might be able to send them to you directly.
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== Apparatus ==
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Email: igem at caltech dot edu
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= Apparatus =
[[Image:Caltech2010Lamp.JPG|thumb|400px|The red/infrared light apparatus]]
[[Image:Caltech2010Lamp.JPG|thumb|400px|The red/infrared light apparatus]]

Latest revision as of 22:01, 27 October 2010


iGEM 2010



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Favorite Bricks & Characterization Data

Heat shock promoter: BBa_K338001

HSP-LacI: BBa_K338002

Plastic, part 1: BBa_K338003

Plastic, part 2: BBa_K338004

All Bricks

Part Name Brick Number Resistance Subparts
phaB1.RBS (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K332023 BBa_K332023] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
Heat-Shock Activated Promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001] Amp Original part
Heat-Shock or IPTG Induced Generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338002 BBa_K338002] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
pLacI.RBS.phaA.RBS (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338003 BBa_K338003] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
phaB1.RBS.phaC1.TT (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338004 BBa_K338004] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
phaA.RBS (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338022 BBa_K338022] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
phaC1 with Double Terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338024 BBa_K338024] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
+OmpR, +(CinR-HSL) Double Promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338029 BBa_K338029] Tet [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
GFP Reporter Device, Heat-Shock Induced [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338042 BBa_K338042] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
+Heat-Shock, +CinR-HSL Double Promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338046 BBa_K338046] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
GFP Reporter Device, Heat-Shock or IPTG Induced [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338062 BBa_K338062] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
Heat-Shock Activated Generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338081 BBa_K338081] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
GFP Reporter Device, Cinr-HSL Induced Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
HSL with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
Constitutive Generator Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
CinR with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
+IPTG, +(CinR-HSL) Double Promoter Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
OmpR Induced Generator Not Submitted Tet [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
HSL.TT.pLacI.p(CinR-HSL) (intermediate) Not Submitted Amp [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
HSL Device, CinR-HSL Induced Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
HSL Device Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
Heat-Shock Activated Generator Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
GFP Reporter Device, OmpR Induced Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
GFP Reporter Device, Constitutive Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
K124014 Holin with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 BBa_K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
K124017 Lysis Cassette with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 BBa_K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
CinR Device, OmpR or CinR-HSL Induced Not Submitted Amp [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
CinR Device, OmpR Induced Not Submitted Amp [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]

If your iGEM team is interested in any of our unsubmitted parts, let us know and we might be able to send them to you directly.

Email: igem at caltech dot edu

Apparatus

The red/infrared light apparatus

The red/infrared light emitting apparatus we built to test our heat shock promoter was modeled after the original [http://openwetware.org/wiki/LightCannon Light Cannon]. It features a theater lamp with a Roscolux #22 Deep Amber red/infrared light filter ([http://www.amazon.com/Rosco-Laboratories-22-ROSCO-Roscolux-22/dp/B000XRCGDE/ref=sr_1_1?ie=UTF8&s=electronics&qid=1287956720&sr=8-1 Amazon], [http://www.rosco.com/includes/filters/SED.asp?titleName=R22:%20Deep%20Amber&imageName=../../images/filters/roscolux/22.jpg Spectrum Data]).

We placed this light on the bench top above a rudimentary stage. By varying the height of the lamp above the stage, we could vary the temperature and light intensity at the stage. In the image, the stage temperature was measured to be 42°C.

Stage of the light apparatus; temperature at 42°C.
 
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