Latest revision as of 21:44, 27 October 2010

Promoters

Subpages

Cadmium Inducible Promoter Team:Nevada/registry submissions

Heavy metal contamination is an important environmental issue. Heavy metals can contaminate soil and water sources in areas where mining and various industrial proccesses have occurred. These metals can then be absorbed into plants which are subsequently eaten by various animals. Heavy metals are usually not excreted readily and are retained within the body of an animal that has consumed a contaminated food source. Cadmium in particular is not excreted readily from the mammals and is known to cause various etiologies stemming from its build-up in organs (Gobe and Cramer). In order to develop a mock cadmium-sensing system in plants the promoter for the Cd-transporter gene AtMRP3 (At3g13080) from A. thaliana was transformed into N. tabacum cells. AtMRP3 is utilized by the plant to sequester Cd2+ in the vacuole, which is thought to prevent the cation from interfering with various biological processes (Bovet et al.). Besides being highly induced by cadmium, AtMRP3 has also shown similar induction patterns when plants were subjected to arsenic or lead, thusly making it a useful sensor for various heavy metal soil contaminants.

AtMRP3 will be the first plant-compatible heavy metal promoter available to the iGEM registry. This promoter could be coupled with a myriad of reporters to indicate whether or not plants are experiencing any type of stress due to the presence of cadmium or other heavy metals.

References
Bovet et al. Transcript levels of AtMRP3 after cadmium treatment: induction of AtMRP3. Plant, Cell and Environment., 26: 371-381, 2003.
Gobe and Cramer. Mitochondria, reactive oxygen species and cadmium toxicity in the kidney. Toxicology Letters., 198: 49-55, 2010.

@@ Line 1: / Line 1: @@
-{{nevadaparts}}
+{{Nevada_css}}
+[[Image:Picture 13.png|border|left|950px]]
+{{Nevada_topbar}}
+<div style="padding: 10px 10px 30px 10px;">
+<p>&nbsp;</p>
 == Promoters ==
@@ Line 8: / Line 14: @@
-{|align="justify"
-|-
 <html>
 <div id="vertmenu">
@@ Line 16: / Line 19: @@
 <ul>
 <li><a href="https://2010.igem.org/Team:Nevada/DREB1C" tabindex="1">DREB 1C</a></li>
-<li><a href="https://2010.igem.org/Team:Nevada/RD29A" tabindex="2">RD29A</a></li>
+<li><a href="https://2010.igem.org/Team:Nevada/RD29A" tabindex="2">rd29A</a></li>
 <li><a href="https://2010.igem.org/Team:Nevada/35S" tabindex="3">35S</a></li>
 <li><a href="https://2010.igem.org/Team:Nevada/CD2Inducible" tabindex="4">CD2+ Inducible</a></li>
@@ Line 22: / Line 25: @@
 </div>
 </html>
+https://static.igem.org/mediawiki/2010/a/aa/Pending.png '''Cadmium Inducible Promoter''' [[Team:Nevada/registry submissions]]
-<p>The 2010 Nevada iGEM team has three goals for this year’s competition.  First, we are going to test the validity of utilizing Nicotiana tabacum protoplasts (NT cells), plant cells without the cell wall, as a model for the expression of higher plant genes for future iGEM competitions.  This system is useful in the respect that the time it takes to obtain transgenic lines of cells is greatly reduced compared to the time to obtain transgenic plants.  <html>
+----
-<a href="https://static.igem.org/mediawiki/2010/7/79/GFP_tobacco_plant.jpg"><img src="https://static.igem.org/mediawiki/2010/7/79/GFP_tobacco_plant.jpg" class="shadow" style="float:left;width:170px;margin:10px"></a>
-</html>These cells can therefore be utilized as a quick proof-of-concept test model before moving synthetic constructs into plants of interest.  We also aim to produce an iGEM-compatible plant-specific plasmid, several stress-inducible plant promoters, reporter genes containing Kozak sequences (ribosome binding sites) and terminators that conform to BioBrick standards.  Lastly, we hope to measure the induction of these stress promoters in real-time by performing a fluorometry assay in which stress will be applied to NT cells and fluorescent output by a reporter (GFP) will be measured to detail induction in real time.  This method has a distinct advantage over microarrays since microarrays are only one ‘snapshot’ in time.</p>
-|
-|}
-<!--- The Mission, Experiments --->
+<html><a href="https://static.igem.org/mediawiki/2010/8/8f/Hileary.jpg"><img src="https://static.igem.org/mediawiki/2010/8/8f/Hileary.jpg" class="shadow" style="float:left;width:200px;margin:10px"></a>
+</html>
+<p>Heavy metal contamination is an important environmental issue.  Heavy metals can contaminate soil and water sources in areas where mining and various industrial proccesses have occurred.  These metals can then be absorbed into plants which are subsequently eaten by various animals.  Heavy metals are usually not excreted readily and are retained within the body of an animal that has consumed a contaminated food source.  Cadmium in particular is not excreted readily from the mammals and is known to cause various etiologies stemming from its build-up in organs (Gobe and Cramer).  In order to develop a mock cadmium-sensing system in plants the promoter for the Cd-transporter gene AtMRP3 (At3g13080) from A. thaliana was transformed into N. tabacum cells.  AtMRP3 is utilized by the plant to sequester Cd2+ in the vacuole, which is thought to prevent the cation from interfering with various biological processes (Bovet et al.).  Besides being highly induced by cadmium, AtMRP3 has also shown similar induction patterns when plants were subjected to arsenic or lead, thusly making it a useful sensor for various heavy metal soil contaminants.</p>
+<p>&nbsp;</p>
+<p>AtMRP3 will be the first plant-compatible heavy metal promoter available to the iGEM registry.  This promoter could be coupled with a myriad of reporters to indicate whether or not plants are experiencing any type of stress due to the presence of cadmium or other heavy metals.</p>
+<br>
+'''References'''
+<br>'''Bovet et al.'''  Transcript levels of AtMRP3 after cadmium treatment: induction of AtMRP3.  Plant, Cell and  Environment., 26: 371-381, 2003.
+<br>'''Gobe and Cramer.'''  Mitochondria, reactive oxygen species and cadmium toxicity in the kidney. Toxicology Letters., 198: 49-55, 2010.
-'''We would like to thank the following sponsors for their support in helping us make this project possible.'''
-Much thanks to the [http://www.cabnr.unr.edu/Students/Majors.aspx Departments of Biochemistry and Biotechnology] and the [http://www.cabnr.unr.edu/Students/Glance.aspx College of Agriculture, Biotechnology and Natural Resources] for their encouragement and support. Thank you [http://www.unr.edu/inbre/ Nevada INBRE] for over $6,000 in support for supplies and registration costs.
-Thank you to [https://asun.unr.edu/Default.aspx Associated Students of the Univeristy of Nevada] for supporting our fund raising efforts.
-Thank you to [http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.] for free enzyme donations.
-Thank you to [http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.] for a discount on our Vector NTI program.
 {| style="color:#FFFFFF;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#008000" width="100%" align="center"
 !align="center"|[[Image:Nevada_CABNR.jpg|200px]]
@@ Line 50: / Line 46: @@
 !align="center"|[[Image:Promega_logo.jpg‎]]
 !align="center"|[[Image:Invitrogen_logo.jpeg]]
+!align="center"|[[Image:Sda logo small.jpg]]
 |}