Team:Brown/Project

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== '''Light-Pattern Controlled Circuit''' ==
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'''Abstract'''
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Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, '''we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light.''' With this production method, '''we can link multiple synthesis steps to a single, clean and rapidly scalable input.'''
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<a href="https://2010.igem.org/Team:Brown/Project/Light_pattern/Overview"><img src="https://static.igem.org/mediawiki/2010/thumb/c/c2/LRCpeektacropped.jpg/780px-LRCpeektacropped.jpg" width="500px"></a>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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== E. Cargo ==
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'''Abstract'''
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Brown_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Brown_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Brown | Team Example]]
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<!--- The Mission, Experiments --->
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We designed a modular Tat-Linker Biobrick that will allow for easy fusion of the Tat-PTD to any other biobricked proteins. Specifically, we aimed to fuse this Tat-Linker in RFC25 format to two bacterial transcription factors, LacI and AraC, with the intent of using the two Tat-TFs as a tool to induce transient gene expression in <i>E. coli</i> without the need to follow through with more time-consuming cell transformation protocols. To test these Tat-TFs, we designed corresponding reporter constructs. We saw a potential application in the portion of the Light-Pattern Controlled Circuit of our project, as well as in any other future genetic circuits that require part-by-part testing.
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!align="center"|[[Team:Brown|Home]]
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<a href="https://2010.igem.org/Team:Brown/Project/Ecargo/Overview"><img src="https://static.igem.org/mediawiki/2010/thumb/8/8d/ECargo.png/400px-ECargo.png" width="500px"></a>
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!align="center"|[[Team:Brown/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Brown Official Team Profile]
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!align="center"|[[Team:Brown/Project|Project]]
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!align="center"|[[Team:Brown/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Brown/Modeling|Modeling]]
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!align="center"|[[Team:Brown/Notebook|Notebook]]
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!align="center"|[[Team:Brown/Safety|Safety]]
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== '''Overall project''' ==
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Your abstract
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 21:07, 27 October 2010


Light-Pattern Controlled Circuit

Abstract

Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light. With this production method, we can link multiple synthesis steps to a single, clean and rapidly scalable input.

E. Cargo

Abstract

We designed a modular Tat-Linker Biobrick that will allow for easy fusion of the Tat-PTD to any other biobricked proteins. Specifically, we aimed to fuse this Tat-Linker in RFC25 format to two bacterial transcription factors, LacI and AraC, with the intent of using the two Tat-TFs as a tool to induce transient gene expression in E. coli without the need to follow through with more time-consuming cell transformation protocols. To test these Tat-TFs, we designed corresponding reporter constructs. We saw a potential application in the portion of the Light-Pattern Controlled Circuit of our project, as well as in any other future genetic circuits that require part-by-part testing.

Retrieved from "http://2010.igem.org/Team:Brown/Project"