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	#mini prep		#mini prep
	#*036①,079①		#*036①,079①
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Latest revision as of 21:04, 27 October 2010

October 10 (Sun)

1. Electrophoresis
2. Ligation
   • 048(C) R1 + FcenA
   • 060(C) 001-2 + GeneⅢ
   • 051(C) R2 + 022
   • 066(A) 3-2P + 026
   • 069(A) 049 + 026
   • 070(A) 052 + 026
3. mini prep
   • 058①,051②,016①,013②
4. cut check
   • 016①,058①,013②,051②,1-13D,050①,025②
   • PCR Products(ADH2,ENO1)
   • Man→034
   • glr→031
   • ADH2→027
   • ENO→029
   • Cel44A→075

1. PCR for DNA segment
   • Cel5B 3/4 α-agultinin
2. To liquid culture
   • 1-13D,1-12M,1-23L,013,056,069-2,061 →culture
3. Transformation
   • 048,060,051,066,069,070,1-13D

October 11 (Mon)

1. Transformation
   • 034,031,027,029,075
   • 014-2,021-2,022-2,025-2,047
2. mini prep
   • 1-23L,1-12M,061①,064-2②,056①②,013①②
   • 1-13D,061②,064-2① →　destruction
3. cut check
   • 1-12M,064-2②,013①,056①,061①,1-23L
4. To liquid culture
   • plate 025(10/8),022(10/8) each 2 colony to culture
5. Electrophoresis
   • 064 ok
   • 061 x
   • 056 x
   • 013 ok
6. Ligation
   • 048(C) R1 + FcenA
   • 051(C) R2 + FcenA
   • 053(C) R2 + 024
   • 059(C) 001-2 + ToR
   • 068(K) 050 + 026
   • 070(A) 052 + 054
   • 072(K) 049 + 054
   • 076(A) 050 + 054
   • 077(A) 2-4A + R1
7. cutcheck
   • R2 →XP
   • 1-23L→ E
8. To liquid culture
   • 1-13D①,070①②,066①②,069①②,060①②,048①②,051①②
9. Electrophoresis
   • 1-23L ok
   • 036② ok
10. Ligation
    • 086(C) 024 + 1-23L
    • 078(A) 2-4A + R2
    • 085(C) 023 + 1-23L
    • 084(K) 049 + 1-23L
    • 083(K) 3-2P + 1-23L
    • 082(K) 035 + 1-23L
    • 081(C) 022 + 1-23L
    • 079(C) 036 + 1-23L

October 12 (Tue)

1. mini prep
   • 1-13D,022-2①②,048①②,060①②,066①②,069①②,070①②
   • 051①→　destrustion
2. Transformation
   • 047,063,069,0071,078,079,080,081,082,083,084,085
3. cutcheck
   • 1-13D,022-2,048,066,069,070,1-13D,060
4. Electrophoresis
   • 1-13D →x
   • 1-13 →x
   • 022-2 →x
   • 048 →OK
   • 060 →?
   • 066 →OK
   • 069 →OK
   • 070 →OK
5. To liquid culture
   • 034,029,027,019-2,022-2,025-2,021-2,031,075,051,072,070,068,053,059,077,076,048,060,1-23L
6. cutcheck
   • 1-23L,048
7. Electrophoresis
8. .Ligation
   • 030(C) SUC d + 1-3A
   • 087(A) 048 + 026
   • 088(A) 048 + 054
   • 089(C) 2-4A + 054

October 13 (Wed)

1. mini prep
   • 027,034,029,053,077,060,025①,059,075,021-2,019-2,022-2,068,076,070,072,1-23L
   • 048、025②→destruction
   • 051→x
2. Treatment of restriction enzyme
   • 027,034,029,053,077,060,025①,059,075,1-23L,021-2,019-2,022-2,068,076,070,072
3. Transformation
   • 030,087,088,089,
4. .Electrophesis
   • 014-2 x
   • 021-2 ok
   • 022 x
   • 025 x
   • 027 x
   • 029 x
   • 034 x
   • 053 ok
   • 077 ok
   • 076 ok
   • 075 ok
   • 072  ?
   • 070 ok
   • 068 ok
   • 060  ?
   • 059  ?
   • 059d
5. .To liquid culture
   • 047①②,063,063,069,071,078,079①②,080,081,082,083,084,085
6. To liquid culture
   • 019-2②,021-2②,025-2②③,027②,029②,034②
7. Ligation
   • 095 2-4A + 072
   • 096 053 + 026
   • 097 053 + 054
   • 098 2-4A + 068
   • 099 075 + 026
   • 070 052 + 054
   • 103 1-12M + 1-23L
   • 093 006 + 1-23L
   • 094 007 + 1-23L
   • 091 009 + 1-23L
   • 102 0-12M + 026
   • 051 R2 + FcenA
   • 105 2-4A + 076

October 14 (Thu)

1. mini prep
   • 019-2,022-2②,025-2②,027,029,034,047①②,069,071,078,080,081,082,083
2. Transformation
3. Treatment of restriction enzyme
   • cut check→R2,022-2②,027②,029②,078,*054①,019-2②,069,071,081,075,082,083,019-2②,039,060,080
   • 054①,019-2②,069,071,081,075,082,083,019-2②,039,060,080
   • for Gel extract→047①②,025-2③,034,1-5A,1-1C,1-3A
4. Electrophoresis
   • R2 ok
   • 019 ok
   • 060 ok
   • 059 ok
   • 071 ok
   • 069 ok
   • 059 ok
   • 078 ok
   • 029 ok
   • 027  ?
   • 022 ok
   • 075 ok
   • 080 x
   • 081 x
   • 082 x
   • 083 x
5. To liquid culture
6. Treatment of restriction enzyme
   • 039②,036①,036②,047①,

October 15 (Fri)

1. mini prep
   • 087①②,088①②,089①②,030①②,079①
   • 051①②,086,085,025-2②,031①～④
2. Treatment of restriction enzyme
   • 079①,087①,088①,089①,030①,030①
3. Ligation
   • 081(A,K) 022 + 081
   • 082(A,K) 035 + 1-23L
   • 083(A,K) 3-2P + 1-23L
   • 092(A,K) 011 + 1-23L
   • 101(A,K) 075 + 1-23L
   • 106(C) 2-4A + 064
   • 108(C) 076 + 075
   • 047(K) 039 + 036
4. Electrophoresis
   • 030 x
   • 089 ok
   • 087 ok
5. 079 ok
   • 036 ok
   • 039 ok
   • glr ok
6. To liquid culture
   • 094,099,102,103,105,51②,085②,091,086,025-2④,098,095,051,093
   • 097 x
   • 096 x
7. Transformation
   • 081,082,083,092,101,047,108,047-2,039,036,106
8. Ligation
   • 031-2(C) glr + 1-3A
   • 100(C) 039 + 079
   • 100-2(A) 039 + 079

October 16 (Sat)

1. Transformation
   • 031-2(C),100(C),100-2(A),BYP5013(A)
2. Ligation
   • 81(C) 2-4A + 087
   • 52 2-4A + 088
   • 098(C) 2-4A + 068
3. Mini prep
   • 051(new),085,086,091,093,094,095,099,102,103,105
4. Transformation
   • 120(C),121(C),098(C),2-4A(A)
5. Treatment of restriction enzyme
   • 2-4A①,051(new),085,086,099,102,103,091,093,094,095,105
6. Electrophoresis
   • 2-4A ok
   • 103 x
   • 102 ok
   • 099 ok
   • 086 ok
   • 085 x
   • 051 x
7. culture
   • 101,083,047,092,081,082,106,108,039,036,098,025-2,082,108,039,036
8. Electrophoresis
   • 091 x
   • 093 ok
   • 094 x
   • 095 x
   • 105 x
9. mini prep
   • 036①,079①

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