From 2010.igem.org
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| - | <br>CLSM IMAGING
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| - | <br>- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution.
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| - | <br>- Spread the samples on the slide with the help of an inoculation loop.
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| - | <br>- Wait for the sample to dry.
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| - | <br>- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide.
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| - | <br>- Observe the slides under Brightfield 100X
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| - | <br>-Settings for GFP:
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| - | <br>HFT 488
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| - | <br>Ch 1
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| - | <br>LP 505
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| - | <br>Line 8
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| - | <br>543 nm excitation
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| - | <br>Laser power 5%
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| - | <br>- Settings for RFP:
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| - | <br>HFT 488/543
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| - | <br>Ch 1
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| - | <br>LP 560
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| - | <br>Line 8
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| - | <br>543 nm excitation
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| - | <br>Laser power 10%
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Revision as of 21:01, 27 October 2010
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