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Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC)
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Cerenseref (Talk | contribs) (New page: <br>CLSM IMAGING <br>- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution. <br>- Spread the samples on the slide with the help of an inoculation loop. ...) |
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<br>- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide. | <br>- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide. | ||
<br>- Observe the slides under Brightfield 100X | <br>- Observe the slides under Brightfield 100X | ||
| - | <br>-Settings for GFP: HFT 488 | + | |
| + | <br>-Settings for GFP: <br>HFT 488 | ||
<br>Ch 1 | <br>Ch 1 | ||
<br>LP 505 | <br>LP 505 | ||
| Line 13: | Line 14: | ||
<br>Laser power 5% | <br>Laser power 5% | ||
| - | <br>- Settings for RFP: HFT 488/543 | + | <br>- Settings for RFP: <br>HFT 488/543 |
<br>Ch 1 | <br>Ch 1 | ||
<br>LP 560 | <br>LP 560 | ||
Revision as of 20:57, 27 October 2010
CLSM IMAGING
- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution.
- Spread the samples on the slide with the help of an inoculation loop.
- Wait for the sample to dry.
- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide.
- Observe the slides under Brightfield 100X
-Settings for GFP:
HFT 488
Ch 1
LP 505
Line 8
543 nm excitation
Laser power 5%
- Settings for RFP:
HFT 488/543
Ch 1
LP 560
Line 8
543 nm excitation
Laser power 10%
