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UTDallas/22 October 2010
From 2010.igem.org
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| + | {{Template:UTDallasTop}} | ||
| + | {{Template:UTDallasMenu}} | ||
| + | <span style="color:#EDE8E8"> | ||
| + | <html> | ||
| + | <style type="text/css"> | ||
| + | table, tr, td {background:transparent; border:0px;} | ||
| + | table table table {background-color:COLOR;} */to change background color from white to transparent | ||
| + | |||
| + | table.calendar { margin: 0; padding: 0px; background-color:#660099} | ||
| + | table.calendar td { margin: 0; padding: 0px; vertical-align: top;} | ||
| + | |||
| + | table.month { margin: 0; padding: 0px; background-color:#E7E5E5} | ||
| + | |||
| + | table.month .heading td { padding:0px; background-color:#660099; color: white; text-align:center; font-size:100%; font-weight:bold; } | ||
| + | table.month .dow td { color:#660099; text-align:center; font-size:90%; } | ||
| + | table.month td.today { background-color:##007D47; } | ||
| + | table.month td { | ||
| + | border: none; | ||
| + | margin: 0; | ||
| + | padding: 0pt 0pt; | ||
| + | font-weight: bold; | ||
| + | font-size: 8 pt; | ||
| + | text-align: center; | ||
| + | background-color: #E7E5E5; | ||
| + | } | ||
| + | #bodyContent table.month a { background:#none; padding:0 } | ||
| + | .day-active { color:#FF8000} | ||
| + | .day-empty { color:#000000} | ||
| + | </style> | ||
| + | </html> | ||
| + | |||
| + | ==Notebook== | ||
| + | |||
| + | {| align="center" | ||
| + | |-align="top" | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=06}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=07}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=08}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=09}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=10}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=11}} | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
===October 22, 2010 === | ===October 22, 2010 === | ||
*Incubated colonies from PyeaR+reporter (pSB1C3) transformations | *Incubated colonies from PyeaR+reporter (pSB1C3) transformations | ||
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*Ligation and transformation with pAlkB + GFP | *Ligation and transformation with pAlkB + GFP | ||
*Incubated colonies from pAlkB + GFP from previous day | *Incubated colonies from pAlkB + GFP from previous day | ||
| + | [[Image:10-22.JPG | 500px | center]] | ||
| + | ;Alkane sensor | ||
| + | *miniprep overnight grown colonies. | ||
| + | *did restriction digestion using XBaI and PstI-HF | ||
| + | *Ran a 2% agarose gel. Nothing seems to be positive | ||
| + | *Again transformed the part E0240 into DH5alpha to do midiprep and get more as the previous one didn't work. | ||
Latest revision as of 20:38, 27 October 2010
Notebook
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October 22, 2010
- Incubated colonies from PyeaR+reporter (pSB1C3) transformations
- Made Chlora plates
- Transformation results showed that ligations did not work
- Digested GFP and conducted gel electrophoresis, gel purification, Dephosphorylation, and PCR purification
- Ligation and transformation with pAlkB + GFP
- Incubated colonies from pAlkB + GFP from previous day
- Alkane sensor
- miniprep overnight grown colonies.
- did restriction digestion using XBaI and PstI-HF
- Ran a 2% agarose gel. Nothing seems to be positive
- Again transformed the part E0240 into DH5alpha to do midiprep and get more as the previous one didn't work.
