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Team:Newcastle/23 June 2010
From 2010.igem.org
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RachelBoyd (Talk | contribs) (→Wednesday Morning) |
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===Wednesday Morning=== | ===Wednesday Morning=== | ||
| + | |||
| + | ====Aims==== | ||
| + | The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone. | ||
| + | |||
| + | ====Equipement List==== | ||
| + | * ''E.coli'' broth culture | ||
| + | * Pipettes | ||
| + | * Plasmid ligation products from yesterday | ||
| + | * Conical flasks | ||
| + | |||
| + | ====Transformation==== | ||
Cells are made competent ready for transformation. | Cells are made competent ready for transformation. | ||
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*Vector | *Vector | ||
*Negative add nothing | *Negative add nothing | ||
| - | *Positive add 1μl uncut | + | *Positive add 1μl uncut |
=== Wednesday afternoon taking a break === | === Wednesday afternoon taking a break === | ||
Revision as of 10:43, 12 July 2010
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Contents |
Wednesday Morning
Aims
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.
Equipement List
- E.coli broth culture
- Pipettes
- Plasmid ligation products from yesterday
- Conical flasks
Transformation
Cells are made competent ready for transformation.
- Positive control(plasmid uncut)- to make sure cells are transfroming properly
- Negative control (just cells)- nothing but competent cells
5 microfuge tubes:
- 1:5
- 1:3
- Vector
- Negative add nothing
- Positive add 1μl uncut
Wednesday afternoon taking a break
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