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Team:Newcastle/21 June 2010
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'''Monday''' | '''Monday''' | ||
===Wet Lab techniques practice=== | ===Wet Lab techniques practice=== | ||
| + | |||
| + | '''Aims''': | ||
| + | The aim of today's Lab training session was to practice aseptic technique and wet lab techniques such as streak plating and broth culture. | ||
| + | |||
| + | '''Equipement list''': | ||
| + | |||
| + | For today's session we needed | ||
| + | |||
| + | * Agar plates | ||
| + | * Pipettes | ||
| + | * Wire loops | ||
| + | * ''E.coli'' (from a colony) | ||
| + | * LB broth | ||
| + | * Bunsen burner | ||
| + | * Conical flasks | ||
| + | * Orbital shaker | ||
| + | |||
'''Tips''': | '''Tips''': | ||
* Use Aseptic technique: Treat everything as a pathogen! | * Use Aseptic technique: Treat everything as a pathogen! | ||
Revision as of 08:51, 12 July 2010
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Monday
Wet Lab techniques practice
Aims: The aim of today's Lab training session was to practice aseptic technique and wet lab techniques such as streak plating and broth culture.
Equipement list:
For today's session we needed
- Agar plates
- Pipettes
- Wire loops
- E.coli (from a colony)
- LB broth
- Bunsen burner
- Conical flasks
- Orbital shaker
Tips:
- Use Aseptic technique: Treat everything as a pathogen!
- Work around the bunsen burner.
- Use a control.
- Clean the bench at the end of the day!
- Heat the wire loop from the middle to the tip.
- Keep plates upside down.
- Keep lids off for as short a time as possible.
- Loosen all the tops off the vessels before you start.
- Flame tops of bottles lightly.
Streak plating
Star plate streaking student, Deena, shows off how it is to be done!
Set up culture for minipreps
5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.
   
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