Team:UPO-Sevilla/Notebook/08 09

From 2010.igem.org

(Difference between revisions)
(New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...)
 
(6 intermediate revisions not shown)
Line 17: Line 17:
       <h1>August, 9th</h1>
       <h1>August, 9th</h1>
-
      <h2>Production Team</h2>
 
-
       <p><strong>David Caballero.</strong> We analyzed plaques spread the day before; it looked like if the bacteria could not grow. There were only three red colonies (negatives) in a plaque. Those devices had promoter and were assembled in high copy vectors. This is why we thought that the high expression of the devices could be harmful for bacteria. We analyzed the digestions in agarose gel to verify it. Al most all the digestions were well made and we only had to repeat two.</p>
+
       <h2>Assembly Team</h2>
-
       <p>In order to avoid that fact again, we assembled devices including promoters in low copy vectors. Like in before cases, we used different vectors depending of the resistance of the origin vectors we need. It is summed up down:</p>
+
       <p>We analyzed plaques spread the day before; it looked like if the bacteria could not grow. There were only three red colonies (negatives) in a plaque. Those devices had promoter and were assembled in high copy vectors. This is why we thought that the high expression of the devices could be harmful for bacteria. We analyzed the digestions in agarose gel to verify it. Almost all the digestions were well made and we only had to repeat two. In order to avoid that fact again, we assembled devices including promoters in low copy vectors. Like in before cases, we used different vectors depending on the resistance of the origin vectors we need. Below it is summed up:</p>
       <ul>
       <ul>
Line 33: Line 32:
       </ul>
       </ul>
-
       <p>It was made the necessary digestions and we left ligation over night at 13ºC on average.</p>
+
       <p>The necessary digestions were made and the ligations were left over night at 13ºC on average.</p>
-
       <h2>Assembly Team</h2>
+
      <p>In other hand, digestion: 2+5+3 with BamI-PstI. We started the following devices (3A method): 7+2+28; 2+6+2+5+3; 2+18; 28+3; 2+4+2+6; 2+28; 18+3: digestion, ligation and transformation.</p>
 +
 
 +
       <h2>DryLab Team</h2>
 +
 
 +
      <p><strong>Modeling: </strong></p>
 +
 
 +
      <p>Simulation issues concerning concentrations' stability fixed. Concentrations behave as expected.</p>
 +
 
 +
      <p>Beginning of <i>E-coli</i> movement modeling, basing on tumbling and straight swim. </p>
 +
 
 +
      <p>First implementation of a random movement is successful. </p>
 +
 
 +
      <p>Simulation of bacterial movement in an environment with Chemoattractants started. Bacteria move towards the concentration gradient, but they tend to move futher away once they reach the highest concentration point. Besides, when bacteria approach a side of the recipient they are in, they usually experience troubles coming back.</p>
 +
 
 +
      <p>By the end of the day, the aforementioned issues are all fixed.</p>
 +
 
 +
 
 +
    <a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_06" title="Go to 6th of August"><span>Go to 6th of August</span></a>
 +
 
 +
    <a class="next_button" href="/Team:UPO-Sevilla/Notebook/08_10" title="Go to 10th of August"><span>Go to 10th of August</span></a>
-
      <p>Digestion: 2+5+3 with BamI-PstI. We started the following devices (3A method): 7+2+28; 2+6+2+5+3; 2+18; 28+3; 2+4+2+6; 2+28; 18+3: digestion, ligation and transformation.</p>
+
    <div class="clear"></div>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>

Latest revision as of 19:59, 27 October 2010

Home Banner

August, 9th

Assembly Team

We analyzed plaques spread the day before; it looked like if the bacteria could not grow. There were only three red colonies (negatives) in a plaque. Those devices had promoter and were assembled in high copy vectors. This is why we thought that the high expression of the devices could be harmful for bacteria. We analyzed the digestions in agarose gel to verify it. Almost all the digestions were well made and we only had to repeat two. In order to avoid that fact again, we assembled devices including promoters in low copy vectors. Like in before cases, we used different vectors depending on the resistance of the origin vectors we need. Below it is summed up:

  • pSB4K5: (1+2) + (4)
  • pSB4C5: (1+2) + (7+2)
  • pSB3T5: (11) + (19) // (12) + (19)
  • pSB3C5: (11+2) + (13+3) // (12+2) + (13+3) // (11+2) + (16+3) // (12+2) + (16+3) // (1+2) + (16+3)

The necessary digestions were made and the ligations were left over night at 13ºC on average.

In other hand, digestion: 2+5+3 with BamI-PstI. We started the following devices (3A method): 7+2+28; 2+6+2+5+3; 2+18; 28+3; 2+4+2+6; 2+28; 18+3: digestion, ligation and transformation.

DryLab Team

Modeling:

Simulation issues concerning concentrations' stability fixed. Concentrations behave as expected.

Beginning of E-coli movement modeling, basing on tumbling and straight swim.

First implementation of a random movement is successful.

Simulation of bacterial movement in an environment with Chemoattractants started. Bacteria move towards the concentration gradient, but they tend to move futher away once they reach the highest concentration point. Besides, when bacteria approach a side of the recipient they are in, they usually experience troubles coming back.

By the end of the day, the aforementioned issues are all fixed.

Go to 6th of August Go to 10th of August
Return to Notebook

Footer

Sponsors

Retrieved from "http://2010.igem.org/Team:UPO-Sevilla/Notebook/08_09"