Team:MIT projectContext

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<dt><b>Phage</b></dt>
<dt><b>Phage</b></dt>
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<dt><b>Mammalian</b></dt>
<dt><b>Mammalian</b></dt>
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">hairy cells and polymerizing phage - context</div></td>
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">project context</div></td>
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<tr><td>
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<br>
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<h4>UV Toggle</h4>
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<hr>
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<br>
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<b>WORK FROM OTHER IGEM TEAMS</b>
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<br>
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<ul>
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<li>
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In 2005, the <a href="https://2006.igem.org/UT_Austin_2005">UT Austin</a> team worked on a light-dependent bacterial printer.
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</li>
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</ul>
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<br>
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<b>RELEVANT LITERATURE</b>
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<ul>
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<li>Kobayashi, Collins, et al.  "Programmable cells: Interfacing natural and engineered gene networks".  PNAS.  2004.</li>
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<li>Crowl, et al.  "Repressor cleavage as a prophage induction mechanism: Hypersensitivity of a mutant λ cI protein to RecA-mediated proteolysis".  J Mol Bio. 1981.</li>
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<li>Gimble and Sauer.  "Mutations in Bacteriophage X Repressor That Prevent RecA-Mediated Cleavage".  Journal of Bacteriology.  1985.</li>
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</ul>
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<br><br>
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<h4>Phage</h4>
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<hr>
<br>
<br>
<b>WORK FROM OTHER IGEM TEAMS</b>
<b>WORK FROM OTHER IGEM TEAMS</b>
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<li>
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In 2006 the McGill team attempted to use leucine zippers fused to split YFP to display on cells and cause the cells to adhere via the split YFP. See: http://parts.mit.edu/wiki/index.php/McGill_University_2006</li>
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In 2006 the <a href="https://2006.igem.org/McGill_University_2006">McGill team</a> attempted to use leucine zippers fused to split YFP to display on cells and cause the cells to adhere via the split YFP.</li>
<br><br>
<br><br>
<li>
<li>
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The 2009 Freiburg Bioware team used Fos/Jun for a 'programmable enzyme' using Fok-fused Fos/Jun as factor in DNA cleavage. See: https://2009.igem.org/Team:Freiburg_bioware/Project/FOS</li>
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The <a href="https://2009.igem.org/Team:Freiburg_bioware/Project/FOS">2009 Freiburg Bioware team</a> used Fos/Jun for a 'programmable enzyme' using Fok-fused Fos/Jun as factor in DNA cleavage.</li>
<br><br>
<br><br>
<li>
<li>
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Paris's team in 2009 attempted to use Jun/Fos as a snare; Jun on signal vesicle, Fos on membrane of receiving cell. See: https://2009.igem.org/Team:Paris#top</li>
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The <a href="https://2009.igem.org/Team:Paris#top"> 2009 Paris team</a> attempted to use Jun/Fos as a snare; Jun on signal vesicle, Fos on membrane of receiving cell.</li>
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<br><br>
<li>
<li>
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This year, Duke's team is using Jun- and Fos-based synthetic leucine zippers for genetic regulation. See: https://2010.igem.org/Team:Duke/Project#Leucine_Zippers
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This year, <a href ="https://2010.igem.org/Team:Duke/Project#Leucine_Zippers">Duke's team </a>is using Jun- and Fos-based synthetic leucine zippers for genetic regulation.</li>
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&larr; <a href="https://2010.igem.org/Team:MIT_phage_results">Results</a>
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<br><br>
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<h4>Mammalian</h4>
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<hr>
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<br>
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<b>WORK FROM OTHER IGEM TEAMS</b>
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<br>
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To our knowledge, no other iGEM team has worked with isolating mechanosensitive promoters and directed osteogenesis.
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<br><br>
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<b>RELEVANT LITERATURE</b>
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<ul>
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<li>Kessler et al.  "Fibroblasts in Mechanically Stressed Collagen Lattices Assume a “Synthetic” Phenotype".  Journal of Biological Chemistry, 2001.</li>
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<li>Silberman et al. "Shear stress-induced transcriptional regulation via hybrid promoters as a potential tool for promoting angiogenesis." Angiogenesis 2009.
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<li>Peake and Haj. "Preliminary characterisation of mechanoresponsive regions of the c-fos promoter in bone cells." FEBS Letters, 2003.
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</ul>
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Latest revision as of 19:53, 27 October 2010

project context

UV Toggle


WORK FROM OTHER IGEM TEAMS
  • In 2005, the UT Austin team worked on a light-dependent bacterial printer.

RELEVANT LITERATURE
  • Kobayashi, Collins, et al. "Programmable cells: Interfacing natural and engineered gene networks". PNAS. 2004.
  • Crowl, et al. "Repressor cleavage as a prophage induction mechanism: Hypersensitivity of a mutant λ cI protein to RecA-mediated proteolysis". J Mol Bio. 1981.
  • Gimble and Sauer. "Mutations in Bacteriophage X Repressor That Prevent RecA-Mediated Cleavage". Journal of Bacteriology. 1985.


Phage


WORK FROM OTHER IGEM TEAMS
The following teams have used some component of our phage system previously. No other teams have used our method of polyphage with incorporated leucine zippers for polymerization (nor is it present in the literature).

  • In 2006 the McGill team attempted to use leucine zippers fused to split YFP to display on cells and cause the cells to adhere via the split YFP.


  • The 2009 Freiburg Bioware team used Fos/Jun for a 'programmable enzyme' using Fok-fused Fos/Jun as factor in DNA cleavage.


  • The 2009 Paris team attempted to use Jun/Fos as a snare; Jun on signal vesicle, Fos on membrane of receiving cell.


  • This year, Duke's team is using Jun- and Fos-based synthetic leucine zippers for genetic regulation.


RELEVANT LITERATURE
  • Rackonjac and Model. "Roles of pIII is Filamentous Phage Assembly". J Mol Bio. 1998.
  • Barbas III, et al. "Phage Display: A Laboratory Manual". CSH Press. 2001.
  • Wang, et al. "Adapter-directed display: a modular design for shuttling display on phage surfaces". J Mol Bio. 2010.
  • Smith and Petrenko. "Phage Display". Chemical Review. 1997.
  • Sweeney, et al. "Assembly of Multimeric Phage Nanostructures Through Leucine Zipper Interactions". Biotechnology and Bioengineering. 2006.
  • Roth, et al. "A Minimized M13 Coat Protein Defines the Requirements for Assembly into the Bacteriophage Particle". J Mol Bio. 2002.
  • Weiss and Sidhu. "Design and Evolution of Artificial M13 Coat Proteins". J Mol Bio. 2000.


Mammalian


WORK FROM OTHER IGEM TEAMS
To our knowledge, no other iGEM team has worked with isolating mechanosensitive promoters and directed osteogenesis.

RELEVANT LITERATURE
  • Kessler et al. "Fibroblasts in Mechanically Stressed Collagen Lattices Assume a “Synthetic” Phenotype". Journal of Biological Chemistry, 2001.
  • Silberman et al. "Shear stress-induced transcriptional regulation via hybrid promoters as a potential tool for promoting angiogenesis." Angiogenesis 2009.
  • Peake and Haj. "Preliminary characterisation of mechanoresponsive regions of the c-fos promoter in bone cells." FEBS Letters, 2003.

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