Team:UNIPV-Pavia/Calendar/September/settimana4
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Screening PCR on 6 colonies picked from I55 was performed. Results were the following: | Screening PCR on 6 colonies picked from I55 was performed. Results were the following: | ||
- | [[Image:UNIPV1020_09_10_I55.jpg|thumb| | + | [[Image:UNIPV1020_09_10_I55.jpg|thumb|200px|center| I55-1..6.]] |
I55-3 seems to be ok. | I55-3 seems to be ok. | ||
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Gel run/cut of samples | Gel run/cut of samples | ||
- | [[Image:UNIPV1021_09_10.jpg|thumb|200px|center| I52( | + | [[Image:UNIPV1021_09_10.jpg|thumb|200px|center| I52(E-X), I53(E-X), I55(E-S), I56(X-P), I57(E-X), I61(E-X).]] |
As you can see only I52, I56, I57 and I61 run correctly (I55 was wrong) so gel extraction was performed on these samples: | As you can see only I52, I56, I57 and I61 run correctly (I55 was wrong) so gel extraction was performed on these samples: | ||
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* I66 = I60(E-S) + I57(E-X) | * I66 = I60(E-S) + I57(E-X) | ||
---- | ---- | ||
- | + | Tecan Test on self-inducible promoters was performed on prepared samples with a different protocol that is to bring the cultures to a known OD. | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
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==September, 23rd== | ==September, 23rd== | ||
- | Digestion of yesterday are repeted (because | + | Digestion of yesterday are repeted (because none of them were postitive) as follows: |
{| border="1" align='center' | {| border="1" align='center' | ||
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Gel run of samples was performed | Gel run of samples was performed | ||
- | [[Image:UNIPV10_23_09_10_MyYeast_digest.jpg|thumb|200px|center| | + | [[Image:UNIPV10_23_09_10_MyYeast_digest.jpg|thumb|200px|center|MyYeast-1/2/3 digest.]] |
All samples seem to be positive!! | All samples seem to be positive!! | ||
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All cultures were grow up to OD=0,1 of Nanodrop. They were then induced with HSL (lysis induction) 100nM | All cultures were grow up to OD=0,1 of Nanodrop. They were then induced with HSL (lysis induction) 100nM | ||
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<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Latest revision as of 19:14, 27 October 2010
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