Latest revision as of 18:19, 27 October 2010

Modeling

Modeling the Quad-state Circuit

Because our light circuit consists of four distinct steady states, we choose to model our system initially using a deterministic system. In this section, we will discuss the following:

The system of ordinary differential equations that we formulated to model our system and the biological basis for each
The parameters we used in our model and the basis for them
The results of our model and the conclusions drawn from them
The illumination device we designed to induce the state switching of our circuit.

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+{{:Team:Brown/templates/header}}
+=Modeling=
+[[Image:Jensmile.jpg|left|300px]]
+===Modeling the Quad-state Circuit===
-==Light Programmable Genetic Circuit==
+Because our light circuit consists of four distinct steady states, we choose to model our system initially using a deterministic system. In this section, we will discuss the following:
+# The [[Team:Brown/Modeling/ODEs | system of ordinary differential equations]] that we formulated to model our system and the biological basis for each
-===Modeling the Quad-state===
+# The [[Team:Brown/Modeling/Parameters | parameters]] we used in our model and the basis for them
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+# The [[Team:Brown/Modeling/Results| results]] of our model and the conclusions drawn from them
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+# The [[Team:Brown/Modeling/Light device| illumination device ]] we designed to induce the state switching of our circuit.
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-The above model demonstrates the interaction between parts that make up the quad-state system. S1, S2, S3, and S4 are reporter molecules that represent each state, and could be replaced by any desired protein (for activation of other pathways, production of enzymes for biosynthesis, etc).
-===470nm illumination device===
-[[Image:Prototype_light_device.jpg]]
-This prototype device uses two super-bright blue LEDs (470nm, 2400mcd) to illuminate cells. It will be used to drive the LOVtap promoter, which is responsive to blue light. The device is controlled by an open-source arduino microcontroller and can be custom-programmed to cycle through various light states for various amounts of time. Source code and schematics for the final device will be posted soon.