Team:MIT mmethods
From 2010.igem.org
(Difference between revisions)
(5 intermediate revisions not shown) | |||
Line 10: | Line 10: | ||
display: block; | display: block; | ||
} | } | ||
+ | #content { | ||
+ | background-image: url('https://static.igem.org/mediawiki/2010/3/3b/Blueflowah.jpg'); | ||
+ | background-attachment: fixed; | ||
+ | } | ||
</style> | </style> | ||
</head> | </head> | ||
Line 15: | Line 19: | ||
<div class="header"> | <div class="header"> | ||
- | <div style="width: | + | |
- | < | + | <div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"> |
- | + | <dl id="nav"> | |
- | + | <dt><b>Bacterial Protocol</b></dt> | |
- | + | <dd> | |
- | + | <ul> | |
- | + | <li><a href="https://2010.igem.org/Team:MIT_bconst">Biobrick Construction</a></li> | |
- | + | <li><a href="https://2010.igem.org/Team:MIT_bexp">Bacterial Experiments</a></li> | |
- | + | </ul> | |
- | </ | + | </dd> |
- | < | + | <dt><b>Mammalian Protocol</b></dt> |
+ | |||
+ | <dd> | ||
+ | <ul> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_mmethods">Microfluidics</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_mge">Genetic Engineering</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_gateway">Gateway Cloning</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </dd> | ||
+ | <dt><b>Phage Protocol</b></dt> | ||
+ | |||
+ | <dd> | ||
+ | <ul> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_phageprot">Basic Protocol</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </dd> | ||
+ | |||
+ | </dl> | ||
</div> | </div> | ||
- | <div id="unique" style="padding: | + | <div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"> |
- | <table width= | + | <table width=650px style="background-color: white; margin-top:5px; padding: 10px;"> |
+ | <tr><td><div class="bodybaby">mammalian microfluidic protocol</div></td> | ||
<tr><td><br>The Mammalian team focused on creating a pressure-sensitive promoter and creating a standard protocol for mammalian genes.<br><br> | <tr><td><br>The Mammalian team focused on creating a pressure-sensitive promoter and creating a standard protocol for mammalian genes.<br><br> | ||
<div class="outline"> | <div class="outline"> |
Latest revision as of 16:54, 27 October 2010
mammalian microfluidic protocol |
The Mammalian team focused on creating a pressure-sensitive promoter and creating a standard protocol for mammalian genes.
1 HTD Preparation Protocol 1.1 PDMS Mixture Preparation 1.2 PDMS Pouring 1.3 PDMS Baking 1.4 PDMS-Device Punching and Bonding 1.5 PDMS Device Bonding 1.6 PDL coating 1.7 Collagen filling 1.8 Cell Seeding 2 Protocol for Deflection Experiments 2.1 Tubing Setup Details 2.2 Adding Medium to Channels 2.3 Connecting device to pressure valve 2.4 Microcontroller details |
HTD Preparation Protocols (adapted from Yannis, Alisha) PDMS Mixture Preparation Materials
Procedure
PDMS Pouring
PDMS Baking
PDMS-Device punching and Bonding
PDMS Device Bonding - Plasma Treatment
PDL Coating
Collagen Filling Material
Procedure
Cell Seeding
Protocol for Deflection Experiments Tubing Setup Details
Adding Media to Channels
Connecting Device to Pressure Valve
Microcontroller details
|