Team:Brown/Protocols

From 2010.igem.org

(Difference between revisions)
(Protocols)
 
(27 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Brown/templates/header}}
{{:Team:Brown/templates/header}}
-
==Protocols==
+
=Protocols=
 +
[[Image:Centrifugespinning.jpg|900px]]
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]].
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]].
-
*PCR
+
[[Image:pipette_macro.jpg|thumb|right|400px]]
-
[[:Team:Brown/Protocols/PCR|PCR]]
+
 
-
*Ligations
+
'''PCR'''
-
[[:Team:Brown/Protocols/Ligation|Ligation]]
+
*[[:Team:Brown/Protocols/PCR|PCR]]
-
*Restriction digests
+
'''PCR Cleanup/Purification'''
-
[[:Team:Brown/Protocols/Restriction_Digest|Restriction Digest]]
+
*[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]]
-
Overnights
+
'''Gel Electrophoresis'''
 +
*[[:Team:Brown/Protocols/Gels|Agarose gels]]
 +
'''Gel Extraction'''
 +
*[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]]
 +
'''Ligations'''
 +
*[[:Team:Brown/Protocols/Ligation|Ligation]]
 +
'''Restriction digests'''
 +
*[[:Team:Brown/Protocols/Restriction_Digest|Restriction [Double] Digest]]
 +
'''Overnights'''
*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]]
*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]]
-
Transformations
+
'''Transformations'''
-
*[[:Team:Brown/Protocols/Transformation|Transformation]]
+
*[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]]
-
Cell competence protocols
+
'''Cell competence protocols'''
-
*[[Media|Protocol_Quick_CaCl2_competence.pdf]]
+
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]]
 +
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]]
 +
'''Minipreps'''
 +
*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]]

Latest revision as of 16:44, 27 October 2010

Protocols

Centrifugespinning.jpg

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.

Pipette macro.jpg

PCR

PCR Cleanup/Purification

Gel Electrophoresis

Gel Extraction

Ligations

Restriction digests

Overnights

Transformations

Cell competence protocols

Minipreps