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Team:Brown/Protocols
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Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]]. | Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]]. | ||
| + | [[Image:pipette_macro.jpg|thumb|right|400px]] | ||
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| + | '''PCR''' | ||
*[[:Team:Brown/Protocols/PCR|PCR]] | *[[:Team:Brown/Protocols/PCR|PCR]] | ||
| - | *[[:Team:Brown/Protocols/Ligation|Ligation] | + | '''PCR Cleanup/Purification''' |
| - | *[[:Team:Brown/Protocols/Restriction_Digest|Restriction Digest] | + | *[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]] |
| - | *[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture] | + | '''Gel Electrophoresis''' |
| - | *[[:Team:Brown/Protocols/Transformation|Transformation] | + | *[[:Team:Brown/Protocols/Gels|Agarose gels]] |
| - | *[[: | + | '''Gel Extraction''' |
| + | *[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]] | ||
| + | '''Ligations''' | ||
| + | *[[:Team:Brown/Protocols/Ligation|Ligation]] | ||
| + | '''Restriction digests''' | ||
| + | *[[:Team:Brown/Protocols/Restriction_Digest|Restriction [Double] Digest]] | ||
| + | '''Overnights''' | ||
| + | *[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | ||
| + | '''Transformations''' | ||
| + | *[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]] | ||
| + | '''Cell competence protocols''' | ||
| + | *[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]] | ||
| + | *[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]] | ||
| + | '''Minipreps''' | ||
| + | *[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]] | ||
Latest revision as of 16:44, 27 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
PCR
PCR Cleanup/Purification
Gel Electrophoresis
Gel Extraction
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols
Minipreps
