Team:Lethbridge
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+ | Check out these important project links! | ||
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+ | <table border="0" width="20%" style="background-color:#000000"> | ||
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+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Judging"> | ||
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- | + | <th><a href="https://2010.igem.org/Team:Lethbridge/Results"> | |
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- | + | <th><a href="https://2010.igem.org/Team:Lethbridge/Collaboration"> | |
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- | + | =<font color="white">Project Description= | |
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+ | The tailings ponds that result from the extraction of heavy crude oil and bitumen (used to make synthetic crude oil) from the oil sands have used up vast amounts of fresh water and contain substantial quantities of useable organic matter. While most of the bitumen is extracted from the oil sands, some is left behind and added to the tailings ponds. The residual hydrocarbon compounds can be potentially extracted from the ponds and utilized as another source of fuel. Consequently, cleaning the tailings ponds and increasing efficiency of extraction from the oil sands. | ||
+ | <br> | ||
- | + | We wish to develop and characterize a BioBrick, that can breakdown some of the more prominent toxic organic compounds found in the tailing ponds to a more useable form. We are currently targeting catechol, a aromatic compound shown to be degraded by bacteria living in the tailings ponds (Kato <i>et al.</i>, 2001). Catechol is being converted into 2-hydroxymuconic semialdehyde, which we later hope to further convert into a useful hydrocarbon compound. | |
- | + | <br> | |
+ | Additionally, we plan to target our <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Catechol_Degradation"><font color="#00DC00">catechol degrading enzyme</font></a></html> into a <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Compartamentalization"><font color="#00DC00"> microcompartment</font></a></html> which the <html><a href="https://2009.igem.org/Team:Lethbridge" target="new"><font color="#00DC00"> Lethbridge 2009</font></a></html> team began the work on. By compartmentalizing the converted catechol, were trying to develop a way of easily removing the useful hydrocarbon product from the tailings ponds. As a proof of principle, we will target the catechol degradation enzyme into the negatively charged microcompartment by the use of a poly-arginine tag. Furthermore, to avoid adding a new species into the oil sands environment we plan on using the <html><a href="https://2010.igem.org/Team:Lethbridge/Project/DNA_Degradation"><font color="#00DC00"> DNA digestion part</font></a></html> created by <html><a href="https://2007.igem.org/Berkeley_UC" target="new"><font color="#00DC00"> Berkley in 2007</font></a></html> to render our <i>Escherichia coli </i>cells unable to reproduce or able to horizontally transfer its genes. | ||
+ | <br> | ||
+ | Finally, we will be continuing to explore the novel method of the mass production of <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Magnetic_Nanoparticles"><font color="#00DC00"> uniform iron nanoparticles</font></a></html>, which is more efficient and cost effective than current methods (Prozorov <i>et al.</i>, 2007). To optimize the production of nanoparticles we are attaching signal peptide sequences to export the protein to different areas of the cell. By attaching these signal peptides and having the protein directed to certain areas of the cell we hope to find which area is most productive to produce nanoparticles. | ||
+ | <br> | ||
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- | + | Reference:<br> | |
+ | Kato, T., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001). Isolation and characterization of psychrotrophic bacteria from oil-reservoir and oil sands. <i>Applied Microbial Biotechnology.</i> 55, 794-800. | ||
+ | <br> | ||
+ | Prozorov, T., Mallapragada, S. K., Narasimhan, B., Wang, L., Palo, P., Nilsen-Hamilton, M., Williams, T. J., Bazylinski, D. A., Prozorov, R., and Canfield, P. C. (2007). Protein-mediated synthesis of uniform superparamagnetic magnetite nanocrystals. Adv. Funct. Mater. Advanced Functional Materials. 17, 951-957 | ||
=<font color="white">Sponsors= | =<font color="white">Sponsors= | ||
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<a href="http://www.see.ualberta.ca/OSRIN.cfm" target="_blank"> | <a href="http://www.see.ualberta.ca/OSRIN.cfm" target="_blank"> | ||
- | <img src="https://static.igem.org/mediawiki/2010/a/a1/UofLOSRINlogo.jpg" width=" | + | <img src="https://static.igem.org/mediawiki/2010/a/a1/UofLOSRINlogo.jpg" width="250"/> |
</a> | </a> | ||
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<th><a href="http://www.iseee.ca" target="_blank"> | <th><a href="http://www.iseee.ca" target="_blank"> | ||
- | <img src="https://static.igem.org/mediawiki/2010/6/6c/UofLISEEElogo.jpg" width=" | + | <img src="https://static.igem.org/mediawiki/2010/6/6c/UofLISEEElogo.jpg" width="550"/> |
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c) Tangible donations of lab supplies or other necessary materials. | c) Tangible donations of lab supplies or other necessary materials. | ||
- | ===Donations can be made in two ways:=== | + | ===<font color="white">Donations can be made in two ways:=== |
1. Donations can be directed towards the U of L iGEM Scholarship account. | 1. Donations can be directed towards the U of L iGEM Scholarship account. | ||
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===<font color="white">Sponsorship Levels=== | ===<font color="white">Sponsorship Levels=== | ||
- | Platinum - $5000+ or gift in kind | + | Platinum - $5000+ or gift in kind <br> |
- | Logo on | + | Logo on team shirts, large logo on scientific poster, large logo on team wiki and verbal recognition during team project presentations/media interviews. |
- | Gold - $2000-$4999 or gift in kind | + | Gold - $2000-$4999 or gift in kind<br> |
Logo on team shirts, medium logo on scientific poster, medium logo on team wiki and written recognition at end of team project presentations. | Logo on team shirts, medium logo on scientific poster, medium logo on team wiki and written recognition at end of team project presentations. | ||
- | Silver - $1000-$1999 or gift in kind | + | Silver - $1000-$1999 or gift in kind<br> |
- | Medium logo on scientific poster, medium logo on team wiki and written recognition at end of team project presentations. | + | Medium logo on team shirts, scientific poster, medium logo on team wiki and written recognition at end of team project presentations. |
- | Bronze - <$999 or gift in kind | + | Bronze - <$999 or gift in kind<br> |
- | Small logo on scientific poster, small logo on team wiki and written recognition at end of team project presentations. | + | Small logo on team shirts, scientific poster, small logo on team wiki and written recognition at end of team project presentations. |
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