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Team:Newcastle/6 July 2010
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Shethharsh08 (Talk | contribs) (→Research) |
Swoodhouse (Talk | contribs) (→Materials and methods) |
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==Materials and methods== | ==Materials and methods== | ||
| - | *''ara'' forward and reverse | + | *''ara'' forward and reverse control primers |
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*Pipettes | *Pipettes | ||
*Universal tubes | *Universal tubes | ||
*Eppendorf tubes | *Eppendorf tubes | ||
*Centrifuge | *Centrifuge | ||
| - | *PCR | + | *PCR |
| - | *Gel electrophoresis | + | *Gel electrophoresis |
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==Protocol== | ==Protocol== | ||
Revision as of 15:04, 27 October 2010
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Contents |
Genomic DNA extraction from Bacillus subtilis strain ATCC 6633
Aim
The aim of this experiment was to extract genomic DNA from Bacillus subtilis strain ATCC 6633. This strain produces the subtilin genes, required for our Subtilin Production and Immunity BioBricks as well as the Quorum sensor biobrick designed by Team Newcastle 2008.
Materials and methods
- ara forward and reverse control primers
- Pipettes
- Universal tubes
- Eppendorf tubes
- Centrifuge
- PCR
- Gel electrophoresis
Protocol
Please refer to: DNA extraction of Bacillus subtilis for materials required and protocol.
Results
Results were as expected see: https://2010.igem.org/Team:Newcastle/7_July_2010
Conclusion
See: https://2010.igem.org/Team:Newcastle/7_July_2010
Research
Today we also continued with our research on filamentous cells which will act as reinforcing fibres in the crack.
- Baarle S van and Bramkamp M. (2010). "The MinCDJ system in Bacillus subtilis prevents minicell formation by promoting divisome disassembly". PloS one. 5(3). e9850.
- Mo AH and Burkholder WF.(2010). "YneA, an SOS-induced inhibitor of cell division in Bacillus subtilis, is regulated posttranslationally and requires the transmembrane region for activity". Journal of bacteriology. 192(12). 3159-73.
   
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