Team:Heidelberg/Modeling/miBSdesigner

(Difference between revisions)

Latest revision as of 13:40, 27 October 2010

BS designer for miRNA

Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8) ?

Customized MISMATCH position

Supplementary region ?

Customized MATCH positions

Modify AU content ?

A in position -1
A in position 1
A in position 9

A in position 0
A in position 8
A in position 10

Sticky ends for integration into plasmid ?

Customized:
5'
3'

Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2

@@ Line 1: / Line 1: @@
-{{:Team:Heidelberg/Single}}
+{{:Team:Heidelberg/Double}}
-{{:Team:Heidelberg/Single_Pagetop|mibsdesigner}}
+{{:Team:Heidelberg/Pagetop|mibsdesigner}}
 <!--<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">-->
 <html>
@@ Line 8: / Line 8: @@
 <style type="text/css">
 .wrapper {width: 260px; text-align:center;}
-.wrapper2 {margin:auto; height: 650px; text-align:left; }
+.wrapper2 {margin:auto; height: 700px; text-align:left; }
 .title {padding-left:0px; line-height:2; font-weight:bold; font-size:17px;}
 .option {padding-left:10px; white-space:pre-wrap;}
@@ Line 120: / Line 120: @@
 		<br>
 			<center>
-				<input value="      Get your Binding Site!      " type="button" onclick="SBS(seqIn)">
+				<input value="      Generate your Binding Site!      " type="button" onclick="SBS(seqIn)">
 			</center>
 	</div>
@@ Line 143: / Line 143: @@
 </div></div>
 </html>
-{{:Team:Heidelberg/Single_Bottom}}
+{{:Team:Heidelberg/Bottom}}