Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

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{{:Team:Heidelberg/Template}}
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{{:Team:Heidelberg/Double}}
{{:Team:Heidelberg/Pagetop|mibsdesigner}}
{{:Team:Heidelberg/Pagetop|mibsdesigner}}
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<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
<style type="text/css">
<style type="text/css">
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div.wrapper {width: 260px; height: 650px; margin:auto; text-align:center;}
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.wrapper {width: 260px; text-align:center;}
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div.wrapper2 {margin:auto; height: 750px; text-align:left; }
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.wrapper2 {margin:auto; height: 700px; text-align:left; }
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div.title {text-indent:30px;}
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.title {padding-left:0px; line-height:2; font-weight:bold; font-size:17px;}
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div.option {text-indent:50px; white-space:pre-wrap;}
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.option {padding-left:10px; white-space:pre-wrap;}
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.optionl {padding-left:10px; white-space:pre-wrap; float:left; font-size:15px;}
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div.optionr {display:inline; white-space:pre-wrap; margin-right: 45px; float:right;}
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.optionr {display:inline; white-space:pre-wrap; margin-right: 45px; float:right; font-size:15px;}
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div.suboption {text-indent:80px;}
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.suboption {padding-left:20px; font-size:15px;}
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div.clear {clear: both;}
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.clear {clear: both;}
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div.reswrap2 {padding:5px; width: 240px; margin:auto; text-align:left; display:none; font-size:12pt;}
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.reswrap2 {padding:5px; width: 240px; margin:auto; text-align:left; display:none; font-size:12pt;}
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.variaresult {font-size: 12pt; color: #11526f; font-weight: bold;}
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.variaresult {font-size: 16px; color: #11526f; font-weight: bold;}
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td {vertical-align:top;}
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<div class="wrapper2">
<div class="wrapper2">
<center>
<center>
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<span style="font-family: Arial; color: #11526f; font-size: 22.0px; text-decoration: underline; font-weight: bold;">
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<div class="t1">
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Construction of microRNA binding sites </span><br><br>
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Construction of microRNA binding sites </div><br>
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<span style="font-size: 17.0px;">Sequence name </span>
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<div style="width:350px; text-align:right;"><span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Input" target="_blank" title="Input help">?</a><span></div>
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<br>
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<div class="largetext" style="width:450px;">
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Sequence name<br>
<input type="text" name="name" id="name" size="40" value="hsa-miR122"/><br>
<input type="text" name="name" id="name" size="40" value="hsa-miR122"/><br>
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<span style="font-size: 17.0px;">microRNA Sequence ( 5' -> 3')</span><br>
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microRNA Sequence ( 5' -> 3')<br>
<input type="text" name="mir" id="mir" size="40" value="UCAGUUUACUAGUGCCAUUUGU"/><br>
<input type="text" name="mir" id="mir" size="40" value="UCAGUUUACUAGUGCCAUUUGU"/><br>
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<span style="font-size: 17.0px;">
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Spacer (inert sequence)<br>
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Spacer (inert sequence) </span><br>
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<input type="text" name="spacer" id="spacer" size="40" value="TTATATTTTATGACA"/>
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<input type="text" name="spacer" id="spacer" size="40" value="TTATATTTTATGACA"/></center>
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</center><br><br>
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<br><br>
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<div class="largetext" style="width:400px;">
<input name="quality" value="perfect" onchange="chMd()" checked="checked" type="radio">
<input name="quality" value="perfect" onchange="chMd()" checked="checked" type="radio">
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<span style="font-size: 17.0px;">
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Perfect Binding Site <br>
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Perfect Binding Site </span>
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<br>
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<input name="quality" value="bulge" onchange="chMd()" type="radio">
<input name="quality" value="bulge" onchange="chMd()" type="radio">
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<span style="font-size: 17.0px;">
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Imperfect Binding Site with 4 nt bulge (9-12)<br>
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Imperfect Binding Site with 4 nt bulge (9-12) </span>
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-
<br>
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<input name="quality" value="personal" onchange="chMd()" type="radio">
<input name="quality" value="personal" onchange="chMd()" type="radio">
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<span style="font-size: 17.0px;">
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Personalized Binding Site  
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Personalized Binding Site </span>
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</div>
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<br>
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<br>
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<br>
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<table>
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<div class="title">
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<tr height=120px>
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<span style="font-size: 17.0px; text-decoration: underline;">
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<td width=290px>
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Seed sequence (1-8) </span></div>
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<div class="title">
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<div class="option"><SELECT name="seed" onchange="chMd1()" disabled="disabled">
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Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Seed_Types" target="_blank" title="Seed sequence help">?</a></span>
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                <OPTION value="0">
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</div>
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<OPTION value="6mer">6mer                (2-7 paired)
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<div class="option"><SELECT name="seed" onchange="chMd1()" disabled="disabled">
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<OPTION value="7merA1">7merA1          (1-7 paired)
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<OPTION value="0">
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<OPTION value="7merm8">7merm8          (2-8 paired)
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<OPTION value="6mer">6mer                (2-7 paired)
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<OPTION value="8mer">8mer                (1-8 paired)
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<OPTION value="7merA1">7merA1          (1-7 paired)
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<OPTION value="custom">Custom
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<OPTION value="7merm8">7merm8          (2-8 paired)
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</SELECT></div>
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<OPTION value="8mer">8mer                (1-8 paired)
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<div class="suboption">
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<OPTION value="custom">Custom
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<span style="font-size: 15.0px;"> Customized </span>
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</SELECT></div>
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<span style="color: #11526f; font-size: 15.0px; font-weight: bold; ">MISMATCH </span>
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<div class="suboption">
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<span style="font-size: 15.0px;">position</span></div>
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Customized <span style="color:#11526f; font-weight:bold;">MISMATCH</span> position
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<div class="suboption">
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<input type="text" id="mis" size="29" disabled="disabled" value="2"/>
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<input type="text" id="mis" size="30" disabled="disabled" value="2"/>
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</div>
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</div>
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</td>
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<br>
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<td>
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<div class="title">
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<div class="title">
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<span style="font-size: 17.0px; text-decoration: underline;">
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Supplementary region &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Supplementary_Region" title="Supplementary region help" target="_blank">?</a></span></div>
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Supplementary region </span></div>
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<div class="option"><SELECT name="comp" onchange="chMd2()" disabled="disabled">
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<div class="option"><SELECT name="comp" onchange="chMd2()" disabled="disabled">
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<OPTION value="0">
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                <OPTION value="0">
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<OPTION value="3">3                  (14-16 paired)
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<OPTION value="3">3                  (14-16 paired)
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<OPTION value="4">4                  (13-16 paired)
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<OPTION value="4">4                  (13-16 paired)
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<OPTION value="5">5                  (13-17 paired)
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<OPTION value="5">5                  (13-17 paired)
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<OPTION value="6">6                  (13-18 paired)
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<OPTION value="6">6                  (13-18 paired)
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<OPTION value="7">7                  (13-19 paired)
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<OPTION value="7">7                  (13-19 paired)
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<OPTION value="8">8                  (13-20 paired)
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<OPTION value="8">8                  (13-20 paired)
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<OPTION value="total">Total            (13-22 paired)
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<OPTION value="total">Total            (13-22 paired)
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<OPTION value="custom">Custom  
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<OPTION value="custom">Custom  
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</SELECT></div>
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</SELECT></div>
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<div class="suboption">
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<div class="suboption">
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Customized <span style="color: #11526f; font-weight:bold;">MATCH</span> positions
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<span style="font-size: 15.0px;"> Customized </span>
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<input type="text" id="match" size="29" disabled="disabled" value="10, 15, 16, 17, 18, 19, 20"/>
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<span style="color: #11526f; font-size: 15.0px; font-weight: bold; ">MATCH </span>
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</div>
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<span style="font-size: 15.0px;">positions (input numbers from 9 to 22)</span></div>
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</td>
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<div class="suboption">
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</tr>
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<input type="text" id="match" size="30" disabled="disabled" value="10, 15, 16, 17, 18, 19, 20"/></div>
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<tr>
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<br>
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<td>
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<br>
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<div class="title">
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<div class="title">
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Modify AU content &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#AU_Content" target="_blank" title="AU help">?</a></span></div>
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<span style="font-size: 17.0px; text-decoration: underline;">
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<table style="line-height:2; font-size:12px;">
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Modify AU content </span>
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<tr>
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</div>
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<td width=130px>
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<div class="optionl"><input name="AU" value="-1" type="checkbox"><span style="font-size: 15.0px;">A in position -1</span></div>
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<input name="AU" value="-1" type="checkbox">A in position -1<br>
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<div class="optionr"><input name="AU" value="0" type="checkbox"><span style="font-size: 15.0px;">A in position 0  </span></div>
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<input name="AU" value="1" disabled="disabled" type="checkbox">A in position 1<br>
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<div class="clear">
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<input name="AU" value="9" disabled="disabled" type="checkbox">A in position 9<br>
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<div class="optionl"><input name="AU" value="1" disabled="disabled" type="checkbox"><span style="font-size: 15.0px;">A in position 1</span></div>
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</td>
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<div class="optionr"><input name="AU" value="8" disabled="disabled" type="checkbox"><span style="font-size: 15.0px;">A in position 8  </span></div></div>
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<td>
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<div class="clear">
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<input name="AU" value="0" type="checkbox">A in position 0<br>
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<div class="optionl"><input name="AU" value="9" disabled="disabled" type="checkbox"><span style="font-size: 15.0px;">A in position 9</span></div>
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<input name="AU" value="8" disabled="disabled" type="checkbox">A in position 8<br>
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<div class="optionr"><input name="AU" value="10" disabled="disabled" type="checkbox"><span style="font-size: 15.0px;">A in position 10</span></div></div><br>
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<input name="AU" value="10" disabled="disabled" type="checkbox">A in position 10<br>
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<br>
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</td>
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<div class="title"><span style="font-size: 17.0px; text-decoration: underline;">Sticky ends for integration in plasmid: </span></div>
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</tr>
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<div class="option">   <SELECT id="stickyends" name="stickyends" onchange="chMd3()">
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</table>
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</td>
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<td>
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<div class="title">Sticky ends for integration into plasmid &nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Sticky_Ends target="_blank" title="ends help"">?</a></span></div></div>
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<div class="option"><SELECT id="stickyends" name="stickyends" onchange="chMd3()">
<OPTION SELECTED value="none">None
<OPTION SELECTED value="none">None
<OPTION value="BBB">Biobricks Standard RFC-12
<OPTION value="BBB">Biobricks Standard RFC-12
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<OPTION value="custom">Custom sequence
<OPTION value="custom">Custom sequence
</SELECT></div>
</SELECT></div>
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<div class="option"><span style="font-size: 15.0px;">     Customized:    5'</span><input type="text" id="end5" size="15" disabled="disabled"/>         <span style="font-size: 15.0px;">3'</span><input type="text" id="end3" size="15" disabled="disabled"/>
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<div class="suboption">Customized:<br>
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5'&nbsp;&nbsp;<input type="text" id="end5" size="15" disabled="disabled"/><br>
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3'&nbsp;&nbsp;<input type="text" id="end3" size="15" disabled="disabled"/>
</div>
</div>
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</td>
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</tr>
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</table>
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<br>
<center>
<center>
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<input value="      Get your Binding Site!      " type="button" onclick="SBS(seqIn)">
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<input value="      Generate your Binding Site!      " type="button" onclick="SBS(seqIn)">
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</center>
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</center>
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<br>
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</div>
</div>
</form>
</form>
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<br>
 
</body>
</body>
<script type="text/javascript" src="https://static.igem.org/mediawiki/2010/3/3a/Very_final_code_miBS_HD2010.txt"> </script>
<script type="text/javascript" src="https://static.igem.org/mediawiki/2010/3/3a/Very_final_code_miBS_HD2010.txt"> </script>
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{{:Team:Heidelberg/Pagemiddle}}
{{:Team:Heidelberg/Pagemiddle}}
<html>
<html>
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<div class="wrapper"><br>
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<div class="wrapper">
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
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<br><br><br><br>
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<br><br>
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<br><br><br>
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<div class="reswrap2" id="resultdiv">
<div class="reswrap2" id="resultdiv">
<div class="variaresult">Name of primer 1</div>
<div class="variaresult">Name of primer 1</div>

Latest revision as of 13:40, 27 October 2010

BS designer for miRNA
Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)              ?
Customized MISMATCH position
Supplementary region        ?
Customized MATCH positions
Modify AU content                ?
A in position -1
A in position 1
A in position 9
 A in position 0
A in position 8
A in position 10
Sticky ends for integration into plasmid   ?
Customized:
5'  
3'  



Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2

Retrieved from "http://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner"