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Team:Tsinghua/Notebook/8 October 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, Group 2c == Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. ---- Double digestion system eGFP 5ul EcoR I 1ul Pst I ...) |
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FD Buffer 2ul | FD Buffer 2ul | ||
H2O 12ul | H2O 12ul | ||
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Kan 4ul | Kan 4ul | ||
EcoR I 1ul | EcoR I 1ul | ||
Revision as of 13:39, 27 October 2010
Module I, Group 2c
Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I.
Double digestion system
eGFP 5ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 11ul
mCherry 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Kan 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Incubate at 37 centidegree overnight.
