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Team:TU Delft/Parts/characterization
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'''Status:''' <partinfo>K398331 Status</partinfo> | '''Status:''' <partinfo>K398331 Status</partinfo> | ||
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[[Image:TU_Delft_Emuls_assay_exp1.jpg|thumb|400px|right|Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the [[Team:TU_Delft/Project/solubility/results|BBa_K398206 characteristics]]]]This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our [[Team:TU_Delft/Project/solubility|solubility]] subproject. | [[Image:TU_Delft_Emuls_assay_exp1.jpg|thumb|400px|right|Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the [[Team:TU_Delft/Project/solubility/results|BBa_K398206 characteristics]]]]This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our [[Team:TU_Delft/Project/solubility|solubility]] subproject. | ||
Revision as of 10:22, 27 October 2010
Characterization
Our favorite parts were also the ones best characterized. This page is a quick overview of the results.
Favorite Parts
[http://partsregistry.org/Part:BBa_K398029 BBa_K398029] - ALDH
Our results suggest that the recombinant strain E. coli 029A functionally express our biobrick [http://partsregistry.org/Part:BBa_K398029 BBa_K398029]. From the statistical analysis that we performed we concluded that the expression of the biobrick [http://partsregistry.org/Part:BBa_K398006 BBa_K398006] under the promoter-rbs combination [http://partsregistry.org/Part:BBa_J13002 BBa_J23100]-[http://partsregistry.org/Part:BBa_J13002 BBa_J61117] increases the dodecanal dehydrogenase activity in E. coli cell extracts 2-fold. Moreover, the enzymatic activities measured for the construct [http://partsregistry.org/Part:BBa_K398029 BBa_K398029] were equivalent to 33.98% of the Pseudomonas putida aldehyde dehydrogenase activity. Read more about it in our results page
View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398331 BBa_K398029 in the parts registry]
Specified Components <partinfo>BBa_K398029 SpecifiedComponents</partinfo>
Designer: <partinfo>BBa_K398029 Designer</partinfo>
Status: <partinfo>BBa_K398029 Status</partinfo>
[http://partsregistry.org/Part:BBa_K398331 BBa_K398331] - pCaif
According to our results, the smallest part that we designed it's working!!!
pCaiF a natural promoter in E. coli that is active at high cAMP levels was characterized attaching E00240 in front of our part. Different GFP production rates were found at the stationary phase showing that GFP production depends on the amount of glucose in the medium. Moreover, growth on a secondary carbon source decreases the GFP production rate.
Read more about our fascinating results in our website!
View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398331 BBa_K398331 in the parts registry]
Specified Components <partinfo>K398331 SpecifiedComponents</partinfo>
Designer: <partinfo>K398331 Designer</partinfo>
Status: <partinfo>K398331 Status</partinfo>
BBa_K398206 - Emulsifier
View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398206 BBa_K398206 in the parts registry]
Specified Components <partinfo>K398206 SpecifiedComponents</partinfo>
Designer: <partinfo>K398206 Designer</partinfo>
Status: <partinfo>K398206 Status</partinfo>
