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Team:Brown/Project/Light pattern/Logic design
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| + | Before we begin our discussion of the circuit logic, let us first review what must be accomplished by this circuit: | ||
| + | *Input | ||
| + | **As explained in the Overview section, we desire the input to the cell to be light. Because light cannot alone activate or repress a promoter, the circuit must in some way be able to translate the light signal to a molecule that can regulate transcription. Additionally, using light as input implies a change in light state - from OFF to ON or vise versa. Thus, the molecule that provides the interface between light and the circuit must be able to switch between active and inactive states. | ||
| + | *Processing | ||
| + | **Given a molecule that toggles between active and inactive, it is easy to imagine a circuit that switches between one state of production and a second. However, we desire to achieve four states! In order to accomplish this, our circuit, unlike the light responsive molecule, cannot return to its original state when the light turns back OFF. Clearly, our circuit must be able to hold "memory." | ||
| + | *Output | ||
| + | **We want our circuit to have distinct states - when possible, we would like a state to turn off after a new state has been turned on (with the exception of the memory, of course!). | ||
Revision as of 10:15, 27 October 2010
Light-Pattern Controlled Circuit
Logic Design
Before we begin our discussion of the circuit logic, let us first review what must be accomplished by this circuit:
- Input
- As explained in the Overview section, we desire the input to the cell to be light. Because light cannot alone activate or repress a promoter, the circuit must in some way be able to translate the light signal to a molecule that can regulate transcription. Additionally, using light as input implies a change in light state - from OFF to ON or vise versa. Thus, the molecule that provides the interface between light and the circuit must be able to switch between active and inactive states.
- Processing
- Given a molecule that toggles between active and inactive, it is easy to imagine a circuit that switches between one state of production and a second. However, we desire to achieve four states! In order to accomplish this, our circuit, unlike the light responsive molecule, cannot return to its original state when the light turns back OFF. Clearly, our circuit must be able to hold "memory."
- Output
- We want our circuit to have distinct states - when possible, we would like a state to turn off after a new state has been turned on (with the exception of the memory, of course!).
