Team:MIT mammalian Switch

From 2010.igem.org

(Difference between revisions)
Line 66: Line 66:
<img src="https://static.igem.org/mediawiki/2010/1/1d/Simplified.png" width=100%>
<img src="https://static.igem.org/mediawiki/2010/1/1d/Simplified.png" width=100%>
<p>
<p>
-
<center>(Click on picture to view larger version)</center>
 
<br>1. Hef1a promoter leads to low level, constitutive expression of the two halves (VgEcR and RxR) of a receptor responsive to the chemical ponasterone A (PonS).
<br>1. Hef1a promoter leads to low level, constitutive expression of the two halves (VgEcR and RxR) of a receptor responsive to the chemical ponasterone A (PonS).
<br>2. VgEcR, RxR, and PonS combine to form an active complex.
<br>2. VgEcR, RxR, and PonS combine to form an active complex.

Revision as of 10:12, 27 October 2010

Synthetic Switch: Implementation of Bistable Toggles in Mammalian Cells
It is useful for a synthetic system capable of sensing mechanical stimuli to be able to convert a transient signal into a constitutive response. During the summer we designed both a complete and a simplified bistable toggle with a low and high output state using positive feedback loops.


1. Hef1a promoter leads to low level, constitutive expression of the two halves (VgEcR and RxR) of a receptor responsive to the chemical ponasterone A (PonS).
2. VgEcR, RxR, and PonS combine to form an active complex.
3. EGSH is induced by the VgECR-RxR-PonS complex.
4. rtTA3 is transcribed after teh activation of EGSH.
5. Each rtTA3 binds to two of the six tetO sites in the TREt promoter and induces the promoter.
6. rtTA, another version of rtTA3, further induces activation of the TREt promoter. This establishes a positive feedback loop, which propels the system from a low to a stable high output state. In the high output state, EYFP is constitutively expressed at a high level.

Retrieved from "http://2010.igem.org/Team:MIT_mammalian_Switch"