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Team:Stanford/NotebookPage/6 July 2010
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#[http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis] | #[http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis] | ||
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| + | =Greg's Lab Notebook= | ||
| + | |||
| + | *During weekly meeting got up to speed with what the team’s been doing while I was away | ||
| + | *Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight: | ||
| + | |||
| + | {| class="wikitable" style="text-align: center; width: 200px; height: 200px;" | ||
| + | |- | ||
| + | ! Function !! Part # !! Distribution Location !! Resistance | ||
| + | |- | ||
| + | | ribosome binding site || B0032 || P1 2I || Amp | ||
| + | |- | ||
| + | | forward double terminator || B0015 || P1 23L || Amp + Kan | ||
| + | |- | ||
| + | | bidirectional double terminator || B0014 || P2 24C || Amp + Kan | ||
| + | |- | ||
| + | | medium constitutive promoter || J23107 || P1 20A || Amp | ||
| + | |- | ||
| + | | strong constitutive promoter || J23100 || P1 18C || Amp | ||
| + | |- | ||
| + | | plasmid backbone || pSB1K3 || P1 5A || Kan | ||
| + | |} | ||
Revision as of 23:09, 8 July 2010
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Alex's References
- [http://www.nature.com/ja/journal/v59/n2/abs/ja200618a.html Self-activation of Serine/Threonine Kinase AfsK on Autophosphorylation at Threonine-168]
- [http://www.ncbi.nlm.nih.gov/pubmed/11952895 afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2).]
Chris's references
- [http://www3.interscience.wiley.com/journal/118634717/abstract?CRETRY=1&SRETRY=0 EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine/threonine kinases and phosphatase in Mycobacterium tuberculosis]
- [http://www.ncbi.nlm.nih.gov/pubmed/18424526 The critical role of embC in Mycobacterium tuberculosis.]
- [http://jb.asm.org/cgi/content/abstract/188/8/2936 Transcriptional Control of the Mycobacterial embCAB Operon by PknH through a Regulatory Protein, EmbR, In Vivo]
- [http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis]
Greg's Lab Notebook
- During weekly meeting got up to speed with what the team’s been doing while I was away
- Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:
| Function | Part # | Distribution Location | Resistance |
|---|---|---|---|
| ribosome binding site | B0032 | P1 2I | Amp |
| forward double terminator | B0015 | P1 23L | Amp + Kan |
| bidirectional double terminator | B0014 | P2 24C | Amp + Kan |
| medium constitutive promoter | J23107 | P1 20A | Amp |
| strong constitutive promoter | J23100 | P1 18C | Amp |
| plasmid backbone | pSB1K3 | P1 5A | Kan |
