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| - | | + | ==Light-Pattern Controlled Circuit== |
| - | ==Light Pattern Controlled Circuit== | + | We don't know how you got here, but you should click below to return to our site: |
| - | | + | [[:Team:Brown/Project/Light_pattern/Overview]] |
| - | __TOC__
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| - | ===Abstract===
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| - | Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, '''we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light.''' With this production method, '''we can link multiple synthesis steps to a single, clean and rapidly scalable input.'''
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| - | ===Overview===
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| - | Our project this year attempts to tackle what we thought to be an emerging issue in synthetic biology. There is a growing trend among iGEM projects towards complex systems; the registry of standard parts is growing quickly and the tools at the disposal of a synthetic biologist are increasing rapidly. While many of these systems rely on an autonomous progression of events in the chasis - say, a cell encounters some environmental stimulus which triggers downstream responses - some require precise user control. This is especially true for projects in the manufacturing area, as often a product is achieved following a progression of steps within the cell.
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| - | ===Workflow/Methods===
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| - | ===Modeling===
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| - | See our modeling page at: blah blah
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| - | ===Results===
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| - | This is some text blah blah
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| - | ===Future Direction===
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