Team:HokkaidoU Japan/Notebook/August26

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=50 ug/uLに濃縮したpSB1C3の[[アガロースゲル電気泳動|電気泳動]]=
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=Electrophoresis of pSB1C3 concentrated to 50 ug/uL=
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[[Image:HokkaidoU Japan 20100826a.jpg‎|200px|right|thumb|]]
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* 昨日DigestionしたpSB1C3 solution 17.4 uLに6x SB 2.8 uL加え，電気泳動した
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[[Image:HokkaidoU Japan 20100826a.jpg‎|200px|right|thumb|Electrophoresis of concentrated pSB1C3]]
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* Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed
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{| class="protocol"
{| class="protocol"
|-
|-
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|レーン
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|'''Lane'''
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|
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|'''DNA'''
|-
|-
|１
|１
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|間違って入れすぎたマーカー
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|Added too much of marker,  mistake
|-
|-
|２
|２
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|λ/''Hin''d III & EcoR I
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|[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png λ/''Hin''d III & EcoR I]
|-
|-
|３
|３
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|}
|}
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* しっかりとDigestion & Ligationされていればダイマー，テトラマーのバンドが見られるはずだが，見られなかった
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* IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
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* 見えているバンドはモノマー（約2000 bp）
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* Only band visible was monomer（about 2000 bp）
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=Filtration of pSB1A3, pSB1C3 and pSB1K3 PCR solutions=
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=pSB1A3, pSB1C3, pSB1K3のPCR solutionろ過=
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Remaining amount from check via electrophoresis，namely 49 uL was filtrated with  Microcon YM-10
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電気泳動で確かめた残り，49 uLをMicrocon YM-10でろ過した．
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Latest revision as of 07:54, 27 October 2010

Notebook

Electrophoresis of pSB1C3 concentrated to 50 ug/uL

Electrophoresis of concentrated pSB1C3
  • Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed
Lane DNA
１ Added too much of marker, mistake
２ λ/Hind III & EcoR I
３ pSB1C3 solution
４ pSB1C3 solution
  • IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
  • Only band visible was monomer（about 2000 bp）

Filtration of pSB1A3, pSB1C3 and pSB1K3 PCR solutions

Remaining amount from check via electrophoresis，namely 49 uL was filtrated with Microcon YM-10
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