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Revision as of 05:13, 27 October 2010 (view source) ThaiPhi (Talk | contribs) (New page: Team:Queens-Canada/29 June 2010) ← Older edit		Revision as of 05:24, 27 October 2010 (view source) ThaiPhi (Talk | contribs) Newer edit →
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-	[[Team:Queens-Canada/29 June 2010]]	+	<h2>June 18, 2010</h2>
		+	<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3>
		+	Hao and Yuli
		+
		+	'''pSip-1''', '''pHsp-3''', '''pUnc-47''', '''pRab-7''', '''pMec-7''' with '''BBa_I714891'''
		+
		+	Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles.
		+	Primers were then added and program was run with annealing temperature of 1 min 15 s.
		+
		+	<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
		+	Hao and Yuli
		+
		+	'''pSip-1'''::'''eGFP''', '''pHsp-3'''::'''eGFP''', '''pMec-7'''::'''eGFP''', '''pUnc-47'''::'''eGFP''', '''pRab-7'''::'''eGFP'''
		+
		+	Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.

---

Revision as of 05:24, 27 October 2010

June 18, 2010

PCR

Hao and Yuli

pSip-1, pHsp-3, pUnc-47, pRab-7, pMec-7 with BBa_I714891

Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles. Primers were then added and program was run with annealing temperature of 1 min 15 s.

Gel Electrophoresis

Hao and Yuli

pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP

Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.