Team:Chiba/Notebook 3

From 2010.igem.org

(Difference between revisions)
Line 209: Line 209:
NFW            5 µL
NFW            5 µL
--------------------
--------------------
 +
　　　　　　　30 µL
--------------------
--------------------
Line 217: Line 218:
NFW            5 µL
NFW            5 µL
--------------------
--------------------
 +
              30 µL
↓
↓
gel extraction
gel extraction
Line 234: Line 236:
NFW                4 µL
NFW                4 µL
-----------------------
-----------------------
 +
                  10 µL
↓
↓
transformation
transformation
Line 248: Line 251:
dH<sub>2</sub>O    4 µL
dH<sub>2</sub>O    4 µL
-----------------------
-----------------------
 +
                  10 µL
↓
↓
transformation
transformation
 +
strain:XL10-Gold
 +
</pre>
</pre>
-
<html><h3>2010-10-13</h3></html>
 
-
<pre>
 
-
Double transformation
 
-
Strain:XL10-Gold
 
-
Plasmid:Plux inv-GFP & Ptrc-LuxR
 
-
 
-
↓
 
-
not Fuluorescence
 
-
</pre>
 
<html><h3>2010-10-21</h3></html>
<html><h3>2010-10-21</h3></html>
Line 270: Line 267:
Strain:DH10B
Strain:DH10B
Plasmid:Plux inv-GFP & Plac-LuxR
Plasmid:Plux inv-GFP & Plac-LuxR
 +
</pre>
<html><h3>2010-10-22</h3></html>
<html><h3>2010-10-22</h3></html>
-
 
+
<pre>
 +
liquid culture
 +
LB (Carb-Kan) 1mL
 +
37℃ 12 h 200 rpm
 +
</pre>
　
　

Revision as of 05:12, 27 October 2010




 



2010-10-8


transformation

BBa_R0061
BBa_E0240


2010-10-9

Mini-prep

BBa_R0061
BBa_E0240

digestion
--------------------
BBa_R0061     20 µL
10×Buffer     3 µL
SpeI　　　　　 1 µL
PstI　　　　　 1 µL
NFW            5 µL
--------------------
　　　　　　　30 µL

--------------------
BBa_E0240      20 µL
10×Buffer      3 µL
XbaI            1 µL
PstI　　　　　  1 µL
NFW             5 µL
--------------------
               30 µL
↓
gel extraction


2010-10-11

Ligation
-----------------------
BBa_R0061 (70 ng)  1 µL       
BBa_E0240 (100 ng) 2 µL
ATP (10 mM)        1 µL
10×Buffer         1 µL
T4 DNA Ligase      1 µL
NFW                4 µL
-----------------------
                  10 µL
↓
transformation

2010-10-12

Ligation
-----------------------
BBa_R0061(100 ng)  1 µL
BBa_E0240 (100 ng) 3 µL
ATP (10 mM)        1 µL
T4 DNA Ligase      1 µL
dH<sub>2</sub>O    4 µL
-----------------------
                  10 µL
↓
transformation
strain:XL10-Gold


2010-10-21

Double transformation

Strain:DH10B
Plasmid:Plux inv-GFP & Plac-LuxR

2010-10-22

liquid culture
LB (Carb-Kan) 1mL
37℃ 12 h 200 rpm


　

Retrieved from "http://2010.igem.org/Team:Chiba/Notebook_3"