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Team:Calgary
From 2010.igem.org
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| - | <img id="boximage1" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuSuffield.png"></img> | + | <img id="boximage1" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuSuffield-1.png"></img> |
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<div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | <div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | ||
| - | <p style="padding-left:10px;"> | + | <p style="padding-left:10px;">What new dangers and unintended consequences will synthetic biology pose to us in the future? iGEM Calgary travels to Defence Research and Development Canada Suffield, a major Canadian Military Research facility that specializes in chemical-biological threats. We presented popular iGEM topics such as biobricks, and the registry of parts to gain the expert’s perspective on potential dangers. <a class="tabLink" href="https://2010.igem.org/Team:Calgary/Community/Suffield">Read more...</a></p> |
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| - | <img id="boximage2" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuLethbridgeWorkshop.png"></img> | + | <img id="boximage2" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuLethbridgeWorkshop-1.png"></img> |
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<div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | <div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | ||
| - | <p style="padding-left:10px;"> | + | <p style="padding-left:10px;">The University of Calgary, Alberta, and Lethbridge iGEM teams met in Lethbridge at a conference put on by Alberta Innovates Technology Futures to learn important aspects associated with iGEM. Workshops included presentation skills, and using accessible language when talking to the media.<a class="tabLink" href="https://2010.igem.org/Team:Calgary/Community/Conferences#lethbridge">Read more...</a></p> |
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| - | <img id="boximage3" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/ | + | <img id="boximage3" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuProteinMan.png"></img> |
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<div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | <div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | ||
| - | <p style="padding-left:10px;"> | + | <p style="padding-left:10px;">Have you ever seen an aggregate protein dance? Look out for iGEM Calgary’s rambunctious mascot “Protein Man” at the Jamboree promoting proper protein expression. <a class="tabLink" href="https://2010.igem.org/Team:Calgary/Extras/Protein_Man">Learn more about him...</a></p> |
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| - | <img id="boximage4" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuBackSale.png"></img> | + | <img id="boximage4" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuBackSale-1.png"></img> |
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<div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | <div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | ||
| - | <p style="padding-left:10px;"> | + | <p style="padding-left:10px;">Team Calgary brings iGEM awareness to the community. The iGEM bake sale was a great success, both in selling cupcakes and educating customers about our team. <a class="tabLink" href="https://2010.igem.org/Team:Calgary/Community">See what else we've been doing...</a></p> |
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| - | <img id="boximage5" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuaGEM.png"></img> | + | <img id="boximage5" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/TabMenuaGEM-1.png"></img> |
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<div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | <div style="height:75px; width:527px; background-image:url('http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Greyportion.png'); float:left; margin-left:-9px;"> | ||
| - | <p style="padding-left:10px;"> | + | <p style="padding-left:10px;">Alberta’s very own Jamboree. The three Alberta iGEM teams, the Universities of Alberta, Calgary, and Lethbridge meet to perform a practice project presentation to each other. Experts within their field are also present to give teams challenging questions, and then meet with each team to suggest improvements.<a class="tabLink" href="https://2010.igem.org/Team:Calgary/Community/Conferences#agem">Read more...</a></p> |
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Revision as of 04:57, 27 October 2010
What new dangers and unintended consequences will synthetic biology pose to us in the future? iGEM Calgary travels to Defence Research and Development Canada Suffield, a major Canadian Military Research facility that specializes in chemical-biological threats. We presented popular iGEM topics such as biobricks, and the registry of parts to gain the expert’s perspective on potential dangers. Read more...
Our modelling project consists of two components: a mathematical model done in MATLAB and an animation done in Autodesk Maya. We hope to model the formation of inclusion bodies, which are aggregations of misfolded protein that can occur within cells. Using Maya, we also hope to visually show other students one of the proposed aggregation mechanisms described in the literature. Read more here...
Our outreach project included educating highschool students about iGEM. Outreach also entailed blog entries and podcast about synthetic life, iGEM and open source as well as Genetically modified foods. The blog entry, podcasts and high school presentations allowed the iGEM students to spread knowledge about iGEM to the general public and to potential future scientists. Read more here...
Many synthetic biology projects involve the expression of recombinant proteins in microorganisms such as E. coli. The problems encountered with many synthetic biology projects often involve problems with protein expression. It is often very difficult to recognize the problem and pinpoint where it lies. The goal of the University of Calgary 2010 iGEM team is to build a protein expression "troubleshooting kit". This kit will contain two systems with which target genes can be inserted. In the resulting cell growth, fluorescent protein production will be used to determine whether there is a problem with protein expression as well as indicate where the protein expression is failing.
Protein expression happens in three steps: the transcription of the DNA to mRNA, the translation of mRNA into an amino acid sequence, and the folding of that amino acid sequence into a protein. Our system detects possible errors in these three steps using two circuits. The first circuit has a fluorescent reporter that is produced when DNA is transcribed into mRNA and another that is produced when mRNA is translated into a functional protein. When both reporter proteins are expressed in the cell, it indicates both transcription and translation are successful. The second circuit involves reporter systems that are activated as a result of protein misfolding. Two native stress-activated promoters from E. coli were engineered upstream to fluorescent reporters that will respond to periplasmic and cytoplasmic protein misfolding. If the protein of interest misfolds in either area of the cell, one of the promoters will be activated and the corresponding fluorescence will be observed.
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