Team:Alberta/Kit components
From 2010.igem.org
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[[Image:Alberta_Spread.jpg|550px|center|thumb|All the components of GENOMIKON can be seen in this picture.]] | [[Image:Alberta_Spread.jpg|550px|center|thumb|All the components of GENOMIKON can be seen in this picture.]] | ||
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{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} |
Revision as of 03:09, 27 October 2010
The DNA Components
Open Reading Frame Parts
Fluorescent Proteins | Carotenoid Pathway | Violacein Pathway | Transcription Factors | Resistance Marker |
---|---|---|---|---|
GFP | CrtE | VioA | LacI | Ampicillin |
mRFP | CrtB | VioB | AraC | Chloramphenicol |
CFP | CrtI | VioD | LambdaCI | Kanamycin |
YFP | CrtY | VioE | TetR | Tetramycin |
Linker Parts
- Transcription
- 5 Anderson Promoters
- Operon Linker
- Anti-linker as a negative control
- Short null-linker for inversion of ends
- Fusion Protein Link
Other Parts
- High, medium and low copy number Oris
- Caps
- Anchors
Other Components
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON.
- Precision Droppers to accurately dispense small volumes of liquids
- Magnetic Racks
- Eppendorf Tubes
- Wash Buffer
- Elution Buffer
- Lyophilized Competent Cells
- LB media
- Plates
- Iron Micro Beads