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Team:Alberta/Kit components
From 2010.igem.org
(Difference between revisions)
| Line 26: | Line 26: | ||
! Resistance Marker | ! Resistance Marker | ||
|- | |- | ||
| - | | | + | | GFP |
| CrtE | | CrtE | ||
| VioA | | VioA | ||
| Line 32: | Line 32: | ||
| Ampicillin | | Ampicillin | ||
|- | |- | ||
| - | | | + | | mRFP |
| CrtB | | CrtB | ||
| VioB | | VioB | ||
| Line 38: | Line 38: | ||
|Chloramphenicol | |Chloramphenicol | ||
|- | |- | ||
| - | | | + | | CFP |
| CrtI | | CrtI | ||
| VioD | | VioD | ||
| Line 44: | Line 44: | ||
| Kanamycin | | Kanamycin | ||
|- | |- | ||
| - | | | + | | YFP |
| CrtY | | CrtY | ||
| VioE | | VioE | ||
Revision as of 02:23, 27 October 2010
The DNA Components
Open Reading Frame Parts
| Fluorescent Proteins | Carotenoid Pathway | Violacein Pathway | Transcription Factors | Resistance Marker |
|---|---|---|---|---|
| GFP | CrtE | VioA | LacI | Ampicillin |
| mRFP | CrtB | VioB | AraC | Chloramphenicol |
| CFP | CrtI | VioD | LambdaCI | Kanamycin |
| YFP | CrtY | VioE | TetR | Tetramycin |
- Transcription
- 5 Anderson Promoters
- Operon Linker
- Anti-linker as a negative control
- Short null-linker for inversion of ends
- Fusion Protein Link
Other Parts
- High, medium and low copy number Oris
- Caps
- Anchors
Other Components
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON.
- Precision Droppers to accurately dispense small volumes of liquids
- Magnetic Racks
- Eppendorf Tubes
- Wash Buffer
- Elution Buffer
- Lyophilized Competent Cells
- LB media
- Plates
- Iron Micro Beads
